Immunoassay reagent for KL-6 assay
The present invention aims to provide an assay reagent and an assay for accurately measuring KL-6, in particular, an assay reagent and an assay for accurately measuring KL-6 in samples containing a rheumatoid factor and/or a nonspecific substance other than the rheumatoid factor. KL-6 in samples that contain a rheumatoid factor and/or a nonspecific substance other than the rheumatoid factor can be accurately measured using an immunoassay reagent comprising a solution at a pH of 4.0 to 5.5 containing a rheumatoid factor interference inhibitor and a solution of an insoluble carrier on which anti-KL-6 antibodies are immobilized.
1. A KL-6 immunoassay reagent kit; comprising:
a first solution at a pH of 4.0 to pH 5.5 containing a rheumatoid factor interference inhibitor; and
a second solution containing an insoluble carrier on which an anti-KL-6 antibody is immobilized.
2. The immunoassay reagent kit according to claim 1 wherein the insoluble carrier is a latex particle.
3. A KL-6 immunoassay comprising:
providing a sample, a rheumatoid factor interference inhibitor, and an insoluble carrier on which an anti-KL-6 antibody is immobilized; and
measuring the change in absorbance accompanying the agglutination of the insoluble carrier due to the immune reaction between KL-6 in the sample and an anti-KL-6 antibody immobilized on the insoluble carrier in a solution at a pH of 4.0 to pH 5.5.
4. A KL-6 immunoassay comprising:
adding to a sample a solution at a pH of 4.0 to pH 5.5 containing a rheumatoid factor interference inhibitor and a solution of solution containing an insoluble carrier on which an anti-KL-6 antibody is immobilized; and
measuring the change in absorbance accompanying the agglutination of the insoluble carrier due to the immune reaction between KL-6 in the sample and the anti-KL-6 antibody immobilized on the insoluble carrier.
5. The immunoassay according to claim 3 or 4 wherein the insoluble carrier is a latex particle.
6. A KL-6 immunoassay comprising:
adding to a sample the first solution according to claim 1 and the second solution according to claim 1 ; and
measuring the change in absorbance accompanying the agglutination of the insoluble carrier due to the immune reaction between KL-6 in the sample and the anti-KL-6 antibody immobilized on the insoluble carrier.
7. The immunoassay according to claim 3 or 6 wherein the insoluble carrier is a latex particle.