Electrochemical detection of capsaicinoid compounds in a sample
An electrochemical method for capsaicinoid and/or related compound detection in a sample by way of a screen printed electrode having a working electrode, a reference electrode, and a counter electrode. The working electrode can be screen-printed using conductive carbon ink; the reference electrode can be screen printed using conductive carbon ink; and the counter electrode can be screen printed using silver/silver chloride. The method includes contacting the electrode with the sample in the presence of electrolyte solution, and determining whether a change in redox potential occurs by way of differential pulse voltammetry, wherein a modulation amplitude is between approximately 0.1 volt/min and approximately 2.0 volt/min, a step potential is between approximately 0.0005 volt and approximately 0.01 volt, a modulation time is 0.05 seconds, and a corresponding interval time is approximately 0.5 second.
1. An electrochemical method for capsaicinoid detection in a sample, the method comprising:
contacting a screen printed electrode having a non-modified working electrode surface with the sample in the presence of an electrolyte solution, wherein said electrolyte solution includes a potassium chloride buffer; and
determining whether a change in redox potential occurs by way of a voltammetry technique.
2. The method according to claim 1 , wherein the capsaiciniod is capsaicin and dihydrocapsaicin.
3. The method according to claim 1 , wherein said electrolyte solution is selected from Britton-Robinson buffer, acetate buffer, phosphate buffer, potassium chloride buffer, methanol, and acetonitrile.
4. The method according to claim 1 , wherein the screen-printed electrode comprises a screen-printed carbon electrode.
5. The method according to claim 1 , wherein the screen printed electrode comprises a screen-printed carbon working electrode and a screen printed silver/silver chloride reference electrode.
6. The method according to claim 1 , wherein the voltammetry technique is selected from cyclic voltammetry (CV), square wave voltammetry (SWV), linear scan voltammetry (LSV), differential pulse voltammetry (DPV), and normal pulse voltammetry (NPV).
7. The method according to claim 6 , wherein the voltammetry technique includes differential pulse voltammetry by way of a modulation amplitude between 0.1 to 2.0 volt/min, a step potential between approximately 0.0005 volt and approximately 0.01 volt, a modulation time of approximately 0.05 second, and a interval time of approximately 0.5 second.