Nucleic acid and corresponding protein named 158P1D7 useful in the treatment and detection of bladder and other cancers
The invention described herein relates to novel nucleic acid sequences and their encoded proteins, referred to as 158P1D7 and variants thereof, and to diagnostic and therapeutic methods and compositions useful in the management of various cancers that express 158P1D7 and variants thereof.
1. An antibody drug conjugate, comprising:
an antibody or antigen binding fragment thereof conjugated to a cytotoxic agent, wherein the antibody or antigen binding fragment thereof specifically binds to a protein consisting of the amino acid sequence of SEQ ID NO: 3, wherein the antibody specifically binds to the protein within amino acid residues 25-45 or 274-285 of SEQ ID NO: 3.
2. The antibody drug conjugate of claim 1 , wherein the antibody or fragment is monoclonal.
3. The antibody drug conjugate of claim 1 , wherein the antibody or fragment is a recombinant protein.
4. The antibody drug conjugate of claim 3 , wherein the antibody or fragment is a single chain monoclonal antibody or fragment thereof.
5. The antibody drug conjugate of claim 1 , wherein the antibody or fragment is an Fab, F(ab′) 2 , or Fv fragment.
6. The antibody drug conjugate of claim 1 , wherein the antibody or fragment is a human antibody or fragment.
7. The antibody drug conjugate of claim 1 , wherein the cytotoxic agent is selected from the group consisting of radioactive isotopes, chemotherapeutic agents, and toxins.
8. The antibody drug conjugate of claim 7 , wherein the cytotoxic agent is a radioactive isotope selected from the group consisting of 211 At, 131 I, 125 I, 90 Y, 186 Re, 188 Re, 153 Sm, 212 Bi, 32 P and radioactive isotopes of Lu.
9. The antibody drug conjugate of claim 7 , wherein the cytotoxic agent is a chemotherapeutic agent selected from the group consisting of paclitaxel, actinomycin, mitomycin, etoposide, tenoposide, vincristine, vinblastine, colchicine, gelonin, and calicheamicin.
10. The antibody drug conjugate of claim 7 , wherein the cytotoxic agent is a toxin selected from the group consisting of diphtheria toxin, enomycin, phenomycin, Pseudomonas exotoxin (PE) A, PE40, abrin, abrin A chain, mitogellin, modeccin A chain, and alpha-sarcin.
11. A pharmaceutical composition that comprises the antibody drug conjugate of claim 1 in a human unit dose form.
12. The pharmaceutical composition of claim 11 , wherein the composition is adapted for cancer treatment.
13. The pharmaceutical composition of claim 12 , wherein the cancer is bladder cancer, colon cancer, pancreatic cancer, ovarian cancer, prostate cancer, or gastric cancer.
14. A method of inhibiting growth of a bladder cancer cell, comprising administering to the cell the antibody drug conjugate of claim 1 .
15. An isolated monoclonal antibody or antigen binding fragment thereof comprising an antigen binding site that specifically binds to amino acid residues 25-45 or 274-285 of SEQ ID NO: 3.
16. An isolated antibody-drug conjugate, comprising:
an antibody or antigen binding fragment thereof conjugated to a cytotoxic agent, wherein the antibody or antigen binding fragment thereof specifically binds to a peptide consisting of amino acid residues 25-45 or 274-285 of SEQ ID NO: 3.