Tumor antigens BFA4 and BCY1 for prevention and / or treatment of cancer
The present invention relates to a nucleic acid encoding a polypeptide and the use of the nucleic acid or polypeptide in preventing and/or treating cancer. In particular, the invention relates to improved vectors for the insertion and expression of foreign genes encoding tumor antigens for use in immunotherapeutic treatment of cancer.
1. An expression vector comprising the nucleic acid consisting of SEQ ID NO.:3.
2. The expression vector of claim 1 wherein the vector is a plasmid or a viral vector.
3. The expression vector of claim 2 wherein the viral vector is selected from the group consisting of poxvirus, alphavirus, adenovirus, retrovirus, herpesvirus, and adeno-associated virus.
4. The expression vector of claim 3 wherein the poxvirus selected from the group consisting of vaccinia, NYVAC, avipox, canarypox, ALVAC, ALVAC(2), fowlpox, and TROVAC.
5. The expression vector of claim 4 wherein the poxvirus selected from the group consisting of NYVAC, ALVAC, and ALVAC(2).
6. An expression vector encoding a polypeptide consisting of SEQ ID NO.:4.
7. The expression vector of claim 6 wherein the vector is a plasmid or a viral vector.
8. The expression vector of claim 7 wherein the viral vector is selected from the group consisting of poxvirus, alphavirus, adenovirus, retrovirus, herpesvirus, and adeno-associated virus.
9. The expression vector of claim 8 wherein the poxvirus selected from the group consisting of vaccinia, NYVAC, avipox, canarypox, ALVAC, ALVAC(2), fowlpox, and TROVAC.
10. The expression vector of claim 9 wherein the poxvirus selected from the group consisting of NYVAC, ALVAC, and ALVAC(2).
11. The expression vector of claim 1 or 6 further comprising at least one nucleic acid encoding a tumor-associated antigen.
12. The expression vector of claim 1 or 6 further comprising at least one nucleic sequence encoding an angiogenesis-associated antigen.
13. The expression vector of claim 1 or 6 further comprising at least one nucleic acid sequence encoding a co-stimulatory component.
14. The expression vector of claim 13 wherein the co-stimulatory component is B7.1.
15. The expression vector of claim 13 wherein the co-stimulatory component is TRICOM comprising B7.1, LFA-3 and ICAM-1.
16. The expression vector of claim 13 wherein the vector is a plasmid or a viral vector.
17. The expression vector of claim 16 wherein the viral vector is selected from the group consisting of poxvirus, alphavirus, adenovirus, retrovirus, herpesvirus, and adeno-associated virus.
18. The expression vector of claim 17 wherein the poxvirus is selected from the group consisting of vaccinia, NYVAC, avipox, canarypox, ALVAC, ALVAC(2), fowlpox, and TROVAC.
19. The expression vector of claim 18 wherein the poxvirus is selected from the group consisting of NYVAC, ALVAC, and ALVAC(2).
20. A pharmaceutical composition comprising an expression vector of claim 1 .
21. A pharmaceutical composition comprising an expression vector of claim 6 .
22. A pharmaceutical composition comprising an expression vector of claim 14 .
23. A pharmaceutical composition comprising an expression vector of claim 16 .
24. A pharmaceutical composition comprising an expression vector of claim 18 .