Neuronal specific targeting of caveolin expression to restore synaptic signaling and improve cognitive function in the neurodegenerative brain and motor function in spinal cord
The invention provides an expression system for producing Caveolin-1 in neuronal cells or neural stem cells comprising a neuron-specific regulatory element and a nucleic acid sequence encoding Caveolin-1.
1. An expression system for producing Caveolin-1 specifically in neuronal cells or neural stem cells for growth and arborization comprising:
(a) a neuron-specific regulatory element; and
(b) a nucleic acid sequence encoding Caveolin-1
wherein the neuron-specific regulatory element of (a) is a promoter and located upstream of the nucleic acid sequence encoding Caveolin-1 of (b) and directs the expression of Caveolin-1 in neuronal cells or neural stem cells for growth and arborization.
2. The expression system of claim 1 , wherein the nucleic acid sequence encoding Caveolin-1 is set forth in SEQ ID NO:1 beginning at position 568 and ending at position 1104.
3. The expression system of claim 1 , wherein the nucleic acid sequence encoding Caveolin-1 is set forth in SEQ ID NO:5 beginning at position 1 and ending at position 537.
4. The expression system of claim 1 , wherein the nucleic acid sequence encoding Caveolin-1 is set forth in SEQ ID NO:7 beginning at position 1 and ending at position 537.
5. The expression system of claim 1 , wherein the neuron-specific regulatory element is the synapsin promoter as set forth in FIG. 8 (SEQ ID NO:1) beginning at position 21 and ending at position 489.