Divergent picornavirus: cosavirus
Presented herein is the discovery of a new human picornavirus, Cosavirus (previously termed Dekavirus), methods of detecting the Cosavirus and diagnosing Cosavirus infection, methods of treating or preventing Cosavirus infection, and methods for identifying anti-Cosavirus compounds.
1. An isolated nucleic acid molecule comprising a sequence that comprises 50 or more contiguous nucleic acids of SEQ ID NO:1, or the complement thereof in a recombinant expression vector.
2. The nucleic acid of claim 1 , which comprises SEQ ID NO:1.
3. The nucleic acid of claim 1 , which comprises SEQ ID NO:9.
4. An isolated and detectably labeled nucleic acid molecule comprising a sequence that comprises 50 or more contiguous nucleic acids of SEQ ID NO:1, or the complement thereof which hybridizes to SEQ ID NO:1 under conditions of hybridization at 42° C. in a solution comprising 50% formamide, 5×SSC, and 1% SDS, and washing at 65° C. in a solution comprising 0.2×SSC and 0.1% SDS.
5. A kit for detecting a Cosavirus nucleic acid that comprises the nucleic acid of claim 4 .
6. A method of detecting a Cosavirus nucleic acid comprising:
(a) contacting a sample suspected of comprising a Cosavirus nucleic acid with a detectably labeled fragment of 12 or more contiguous nucleic acids of SEQ ID NO:1 or the complement thereof bound to a solid support, and
(b) detecting the presence or absence of hybridization.
7. A method of detecting a Cosavirus nucleic acid comprising:
(a) amplifying nucleic acid of a sample suspected of containing a Cosavirus with a primer pair that is a fragment of 12 or more contiguous nucleic acids of SEQ ID NO:1 or the complement thereof producing a partially double stranded nucleic acid molecule prior to amplification followed by producing, an amplification product; and
(b) detecting the presence of an amplification product, thereby detecting the presence of the Cosavirus nucleic acid.
8. An isolated nucleic acid that consists of 25 or more contiguous nucleic acids of SEQ ID NO:1, or the complement thereof in a recombinant vector.
9. An isolated nucleic acid molecule comprising SEQ ID NO:1, or the complement thereof in a recombinant vector.
10. The expression vector of any of claims 8 to 9 , which encodes the protein of SEQ ID NO:2.
11. An isolated host cell comprising the expression vector of any of claims 8 to 9 .
12. An isolated nucleic acid molecule comprising a nucleotide sequence at least 80% identical to SEQ ID NO:1, or the complement thereof in a recombinant vector.
13. An isolated nucleic acid molecule comprising a nucleotide sequence at least 90% identical to SEQ ID NO:1, or the complement thereof in a recombinant vector.
14. An isolated nucleic acid molecule comprising a nucleotide sequence at least 95% identical to SEQ ID NO:1, or the complement thereof in a recombinant vector.