Lipid membrane structure having intranuclear migrating property
A lipid membrane structure for delivering a substance into a nucleus of a cell, wherein lipid membrane is modified with (a) a polypeptide comprising the amino acid sequence of SEQ ID NO: 1, and/or (b) a polypeptide consisting of an amino acid sequence comprising the amino acid sequence of SEQ ID NO: 1, but including deletion and/or substitution and/or insertion of one or several amino acid residues, and having an activity of promoting migration of the lipid membrane structure into a nucleus of a cell, which can efficiently deliver a nucleic acid into a nucleus of an immunocyte such as dendritic cell.
1. A lipid membrane structure for delivering a substance into a nucleus of a cell that encapsulates the substance to be delivered and that is modified with a polypeptide having amino acid residues which consist of the amino acid residues in the amino acid sequence of SEQ ID NO: 1.
2. The lipid membrane structure according to claim 1 , wherein the lipid membrane structure is a liposome.
3. The lipid membrane structure according to claim 1 , wherein the polypeptide is modified with a hydrophobic group, and the hydrophobic group is inserted into the lipid membrane.
4. The lipid membrane structure according to claim 1 , which has a polypeptide containing a plurality of contiguous arginine residues on the surface.
5. The lipid membrane structure according to claim 1 , which has a polyalkylene glycol on the surface.
6. The lipid membrane structure according to claim 1 , wherein a nucleic acid and a cationic polymer are enclosed in the inside of the lipid membrane structure.
7. The lipid membrane structure according to claim 1 , wherein the substance to be delivered is a nucleic acid encoding an antigen polypeptide to be presented on the surface of an immunocyte.
8. The lipid membrane structure according to claim 7 , wherein the nucleic acid is DNA, and the DNA does not contain CpG.
9. A pharmaceutical composition containing the lipid membrane structure according to claim 1 as an active ingredient.
10. A polypeptide
having amino acid residues which consist of the amino acid residues in the amino acid sequence of SEQ ID NO: 1.