System and method for measuring fluorescence of a sample
View Patent ↗The present disclosure provides a system and a method for measuring fluorescence of a sample. The sample may be a polymerase-chain-reaction (PCR) array, a loop-mediated-isothermal amplification array, etc. LEDs are used to excite the sample, and a photodiode is used to collect the sample's fluorescence. An electronic offset signal is used to reduce the effects of background fluorescence and the noises from the measurement system. An integrator integrates the difference between the output of the photodiode and the electronic offset signal over a given period of time. The resulting integral is then converted into digital domain for further processing and storage.
1. An electronic system for measuring fluorescence of a sample, comprising
a detector adapted to detect fluorescence emitted from the sample;
an offset adjustment unit; and
an integrator adapted to receive an output of the detector and an output of the offset adjustment unit and to provide an integrator output;
wherein
the output of the detector is proportional to fluorescence intensities detected by the detector;
the integrator output is proportional to integration of a difference between the output of the offset adjustment unit and the output of the detector over a period of time; and
the output of the offset adjustment unit is adapted to reduce effects of background fluorescence and electronic noises on the integrator output.
2. The system of claim 1 , further comprising an excitation source adapted to irradiate the sample with electromagnetic waves that induce the sample's fluorescence emissions.
3. The system of claim 1 , further comprising
an excitation source adapted to irradiate the sample with electromagnetic waves that induce the sample's fluorescence emissions,
wherein the excitation source comprises a light-emitting diode (LED) or a laser diode.
4. The system of claim 1 , wherein the detector comprises a photodiode, a charge-coupled device, or a photomultiplier tube.
5. The system of claim 1 , further comprising a microcontroller adapted to adjust the output of the offset adjustment unit.
6. The system of claim 1 , further comprising
a microcontroller adapted to adjust the output of the offset adjustment unit,
wherein
the offset adjustment unit comprises a digital-to-analog converter (DAC) adapted to convert an offset control signal from the microcontroller to an analog offset signal; and
the output of the offset adjustment unit is adapted to vary with the analog offset signal.
7. The system of claim 1 , wherein the output of the offset adjustment unit is a constant.
8. The system of claim 1 , wherein the output of the offset adjustment unit is a constant such that
the output of the integrator is zero or close to zero when no electromagnetic wave from an excitation source is irradiated on the sample.
9. The system of claim 1 , wherein the output of the offset adjustment unit is a constant such that
the output of the integrator is zero or close to zero before any reaction takes place in the sample.
10. The system of claim 1 , further comprising a digital display.
11. The system of claim 1 , further comprising a digital display, wherein the digital display comprises a seven-segment LED display.
12. The system of claim 1 , wherein the integrator comprises a trans-impedance amplifier.
13. The system of claim 1 , wherein the integrator comprises a capacitor and an operational amplifier.
14. The system of claim 1 , wherein the sample comprises a loop-mediated isothermal amplification assay or a polymer-chain-reaction assay.
15. The system of claim 1 , further comprising a temperature control unit that is adapted to maintain the sample at a given temperature.
16. The system of claim 1 , further comprising
a microcontroller; and
a temperature control unit adapted to maintain the sample at a given temperature, comprising a temperature sensor, and a power amplifier, and a digital-to-analog converter (DAC),
wherein
the DAC is adapted to receives inputs from the microcontroller and sends outputs to the power amplifier; and
the power amplifier is connected to a heater that is adapted to heat the measured sample.
17. The system of claim 1 , further comprising
a microcontroller; and
a temperature control unit adapted to maintain the sample at a given temperature, comprising a temperature sensor, and a power amplifier, and a digital-to-analog converter (DAC),
wherein
the DAC is adapted to receive inputs from the microcontroller and sends outputs to the power amplifier;
the power amplifier is connected to a heater that is adapted to heat the measured sample; and
the microcontroller is adapted to control the temperature control unit according to a PID control algorithm.
18. A measurement system, comprising
the electronic system of claim 1 ;
a vial containing the sample and located close to the detector of the electronic system;
a heater adapted to maintain the sample at a given temperature; and
insulation foam covering the heater and the vial.
19. The system of claim 1 , further comprising
a microcontroller,
a temperature control unit configured to raise the temperature of the sample to a target reaction temperature and to maintain the sample at the target reaction temperature,
wherein
the microcontroller is configured to automatically adjust the output of the offset adjustment unit in such a way that the output of the integrator is zero or close to zero until start of a reaction in the measured sample.
20. The system of claim 19 , wherein the microcontroller is configured to maintain the output of the offset adjustment unit at a constant value throughout measurement of the fluorescence emitted from the sample.