Method and apparatus for measuring particle characteristics through mass detection
Method for measuring a target particle property. A suspended microchannel resonator is calibrated to determine the relationship between a detected mass and a resonance frequency shift of the resonator. The target particle is suspended in a fluid and introduced into the resonator, and the resonator frequency shift due to the particle is measured. Target particle mass is calculated from the resonator frequency shift, the target particle density, and the fluid density. A target particle property such as size or volume is determined from the calculated target particle mass.
1. Method for measuring a property of a target particle comprising:
calibrating a suspended microchannel resonator to determine the relationship between the resonance frequency of the resonator and the detected mass due to the presence of a particle inside the resonator using at least one of particles of known mass and density and fluids of known density;
introducing a single target particle into the resonator along with a fluid at a particle/fluid volume concentration near or less than 1/(resonator volume) and measuring the shift in resonance frequency; and
calculating a property of the single target particle from the shift in resonance frequency.
2. The method of claim 1 wherein the property is mass and mass is calculated by the equation
particle mass =detected mass/(1 −fluid density/particle density).
3. The method of claim 1 wherein the property is volume and volume is calculated by the equation
particle volume =detected mass/(fluid density−particle density).
4. Method of claim 1 wherein the property is diameter and diameter is determined by the relationship diameter=CubeRoot(6 ×detected mass/(Pi*(fluid density−particle density))).
5. The method of claim 1 wherein the property is linear size and linear size is determined using relations between volume and size for selected shapes.
6. Method of claim 1 wherein the target particles are droplets in an emulsion.
7. Method of claim 1 wherein the target particles are cells.