Isolation and use of FAD2 and FAE1 from
The present invention provides isolated FAD2 and FAE1 genes and FAD2 and FAE1 protein sequences of Camelina species, e.g., Camelina sativa , mutations in Camelina FAD2 and FAE1 genes, and methods of using the same. In addition, methods of altering Camelina seed composition and/or improving Camelina seed oil quality are disclosed. Furthermore, methods of breeding Camelina cultivars and/or other closely related species to produce plants having altered or improved seed oil and/or meal quality are provided.
1. A method of altering and/or improving fatty acid composition of a Camelina plant cell, plant part, tissue culture or whole plant, the method comprising disrupting one, two, or three copies of FAD2 genes and/or one, two or three copies of FAE1 genes in said Camelina plant cell, plant part, tissue culture or whole plant.
2. The method of claim 1 , wherein the disruption is achieved by one or more mutations selected from the mutations listed in Tables 7 to 12 for a particular FAD2 or FAE1 gene.
3. A method of breeding Camelina plants to produce altered levels of fatty acids in seed oil and/or meal, wherein the method comprises
i) making a cross between a Camelina plant with one or more mutations listed in Tables 7-12 with a second Camelina plant to produce an F1 plant;
ii) backcrossing the F1 plant to the second Camelina plant; and
iii) repeating the backcrossing step to generate a near isogenic line, wherein the one or more mutations are integrated into the genome of the second Camelina plant; wherein the near isogenic line derived from the second Camelina plant with the integrated mutations has altered seed oil composition compared to that of the second Camelina plant without the integrated mutations; wherein the mutations disrupt two or three homologous copies of endogenous FAD2 and/or two or three homologous copies of FAE1 genes.
4. The method of claim 3 , wherein the near isogenic line has an increased oleic acid (18:1) level, and/or reduced polyunsaturated fatty acids level and/or reduced very long chain fatty acids (C>18) level in the seed oil and/or meal compared to that of the second Camelina plant without the integrated mutation or mutations.