Protection of virus particles from phagocytosis by expression of CD47
View Patent ↗The present invention relates to a viral particle. The viral particle has a radius of less than about 1 μm, and at least one peptide comprising at least a biologically active portion of CD47. The present invention also includes a method of increasing the life of a particle in vivo in a mammal. The method includes the steps of expressing at least one peptide comprising at least a biologically active portion of CD47 in a viral particle, and administering the viral particle having CD47 expressed to a mammal, wherein the administered viral particle has a longer half life in the mammal than an otherwise identical viral particle that does not have CD47 expressed thereon.
1. A composition comprising:
a viral particle having a radius of less than about 400 nm; and,
at least one peptide comprising SEQ ID NO:2,
wherein said at least one peptide is expressed on an exposed surface of said viral particle.
2. The composition of claim 1 , wherein said particle has a radius of less than about 150 nm.
3. The composition of claim 1 , wherein said particle comprises a lipid coat.
4. The composition of claim 3 , wherein said particle is a lentivirus.
5. The composition of claim 1 , wherein the amount of said peptide comprising SEQ ID NO:2 in said viral particle is between about 20 and about 250 molecules/μm 2.
6. A pharmaceutical composition comprising:
a viral particle having a radius of less than about 400 nm; and,
at least one peptide comprising SEQ ID NO:2,
wherein said at least one peptide is expressed on an exposed surface of said viral particle; and,
a pharmaceutically acceptable carrier.
7. A method of preparing a viral particle that evades phagocytosis by a phagocytic cell, said method comprising expressing at least one peptide comprising SEQ ID NO:2 on an exposed surface of said viral particle,
whereby said viral particle evades phagocytosis by said phagocytic cell when said viral particle is exposed to said phagocytic cell.
8. A method of increasing the life of a viral particle in vivo in a mammal, said method comprising expressing at least one peptide comprising SEQ ID NO:2 on an exposed surface of said viral particle and administering said viral particle having at least one peptide comprising SEQ ID NO:2 expressed to said mammal,
wherein said administered viral particle has a longer half life in said mammal than an otherwise identical viral particle that does not have at least one peptide comprising SEQ ID NO:2 expressed thereon.
9. The method of claim 8 , wherein said viral particle has a radius of less than about 400 nm.
10. The method of claim 9 , wherein said viral particle has a radius of less than about 150 nm.
11. The method of claim 8 , wherein said viral particle comprises a lipid coat.
12. The method of claim 11 , wherein said viral particle is a lentivirus.
13. The method of claim 8 , wherein the amount of said peptide comprising SEQ ID NO:2 in said viral particle is between about 20 and about 250 molecules/μm 2 .
14. The method of claim 8 , wherein said at least one peptide comprising SEQ ID NO:2 is human CD47.