Polypeptides having endoglucanase activity and polynucleotides encoding same
The present invention relates to isolated polypeptides having endoglucanase activity and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.
1. A nucleic acid construct comprising a polynucleotide encoding a polypeptide having endoglucanase activity, wherein the polynucleotide is operably linked to one or more heterologous control sequences that direct production of the polypeptide in an expression host, and wherein the polypeptide has at least 95% sequence identity to the sequence of amino acids 20-430 of SEQ ID NO: 4, or is a fragment of the sequence of amino acids 20-430 of SEQ ID NO: 4.
2. The nucleic acid construct of claim 1 , wherein the polypeptide having endoglucanase activity has at least 95% sequence identity to the sequence of amino acids 20-430 of SEQ ID NO: 4.
3. The nucleic acid construct of claim 1 , wherein the polypeptide having endoglucanase activity has at least 97% sequence identity to the sequence of amino acids 20-430 of SEQ ID NO: 4.
4. The nucleic acid construct of claim 1 , wherein the polypeptide having endoglucanase activity is a variant of the sequence of amino acids 20-430 of SEQ ID NO: 4, comprising a substitution, deletion, and/or insertion of one or more amino acids.
5. The nucleic acid construct of claim 1 , wherein the polypeptide having endoglucanase activity comprises the sequence of amino acids 20-430 of SEQ ID NO: 4.
6. The nucleic acid construct of claim 1 , wherein the polypeptide having endoglucanase activity is encoded by a polynucleotide that hybridizes with nucleotides 58 to 1740 of SEQ ID NO: 3, or the cDNA sequence thereof under very high stringency conditions, wherein very high stringency conditions are defined as prehybridization and hybridization at 42° C. in 5×SSPE, 0.3% SDS, 200 μg/ml sheared and denatured salmon sperm DNA, and 50% formamide and washing three times each for 15 minutes using 2×SSC, 0.2% SDS at 70° C.
7. The nucleic acid construct of claim 1 , wherein the polypeptide having endoglucanase activity further comprises a cellulose binding domain.
8. A process for degrading a cellulosic material, said process comprising:
(i) treating the cellulosic material with an enzyme composition, wherein the composition comprises a polypeptide having endoqlucanase activity; and
(ii) recovering the degraded cellulosic material;
wherein the polypeptide having endoglucanase activity has at least 95% sequence identity to the sequence of amino acids 20-430 of SEQ ID NO: 4, or is a fragment of the sequence of amino acids 20-430 of SEQ ID NO: 4.
9. A process for producing a fermentation product, said process comprising:
(a) saccharifying a cellulosic material with an enzyme composition, wherein the composition comprises a polypeptide having endoglucanase activity;
(b) fermenting the saccharified cellulosic material with one or more fermenting microorganisms to produce the fermentation product; and
(c) recovering the fermentation product from the fermentation;
wherein the polypeptide having endoglucanase activity has at least 95% sequence identity to the sequence of amino acids 20-430 of SEQ ID NO: 4, or is a fragment of the sequence of amino acids 20-430 of SEQ ID NO: 4.
10. A nucleic acid construct comprising a polynucleotide encoding a polypeptide having a catalytic domain, wherein the polynucleotide is operably linked to one or more heteroloqous control sequences that direct production of the polypeptide in an expression host, wherein the catalytic domain has at least 95% sequence identity to the sequence of amino acids 100-430 of SEQ ID NO: 4 or comprises a fragment of the sequence of amino acids 100-430 of SEQ ID NO: 4, and wherein the catalytic domain has endoglucanase activity.
11. The nucleic acid construct of claim 10 , wherein the polypeptide further comprises a cellulose binding domain.
12. A process for degrading a cellulosic material, said process comprising:
(i) treating the cellulosic material with an enzyme composition, wherein the composition comprises a polypeptide having endoglucanase activity; and
(ii) recovering the degraded cellulosic material;
wherein the polypeptide having endoglucanase activity comprises a catalytic domain having at least 95% sequence identity to the sequence of amino acids 100-430 of SEQ ID NO: 4, or a fragment of the sequence of amino acids 100-430 of SEQ ID NO: 4.
13. A process for producing a fermentation product, said process comprising:
(a) saccharifying a cellulosic material with an enzyme composition, wherein the composition comprises a polypeptide having endoglucanase activity;
(b) fermenting the saccharified cellulosic material with one or more fermenting microorganisms to produce the fermentation product; and
(c) recovering the fermentation product from the fermentation;
wherein the polypeptide having endoglucanase activity comprises a catalytic domain having at least 95% sequence identity to the sequence of amino acids 100-430 of SEQ ID NO: 4, or a fragment of the sequence of amino acids 100-430 of SEQ ID NO: 4.
14. An expression vector comprising the nucleic acid construct of claim 1 .
15. An isolated recombinant host cell comprising the nucleic acid construct of claim 1 .
16. The nucleic acid construct of claim 10 , wherein the catalytic domain has at least 95% sequence identity to the sequence of amino acids 100-430 of SEQ ID NO: 4.
17. The nucleic acid construct of claim 10 , wherein the catalytic domain has at least 97% sequence identity to the sequence of amino acids 100-430 of SEQ ID NO: 4.
18. The nucleic acid construct of claim 10 , wherein the catalytic domain comprises amino acids 100-430 of SEQ ID NO: 4.
19. The process of claim 8 , wherein the polypeptide having endoglucanase activity has at least 97% sequence identity to the sequence of amino acids 20-430 of SEQ ID NO: 4.
20. The process of claim 8 , wherein the polypeptide having endoglucanase activity comprises the sequence of amino acids 20-430 of SEQ ID NO: 4.
21. The process of claim 8 , wherein the composition further comprises a cellobiohydrolase, a beta-glucosidase, and a GH61 polypeptide having cellulolytic enhancing activity.
22. The process of claim 9 , wherein the polypeptide having endoglucanase activity has at least 97% sequence identity to the sequence of amino acids 20-430 of SEQ ID NO: 4.
23. The process of claim 9 , wherein the polypeptide having endoglucanase activity comprises the sequence of amino acids 20-430 of SEQ ID NO: 4.
24. The process of claim 9 , wherein the composition further comprises a cellobiohydrolase, a beta-glucosidase, and a GH61 polypeptide having cellulolytic enhancing activity.
25. The process of claim 12 , wherein the polypeptide having endoglucanase activity comprises a catalytic domain having at least 97% sequence identity to the sequence of amino acids 100-430 of SEQ ID NO: 4.
26. The process of claim 12 , wherein the polypeptide having endoglucanase activity comprises the sequence of amino acids 100-430 of SEQ ID NO: 4.
27. The process of claim 12 , wherein the composition further comprises a cellobiohydrolase, a beta-glucosidase, and a GH61 polypeptide having cellulolytic enhancing activity.
28. The process of claim 13 , wherein the polypeptide having endoglucanase activity comprises a catalytic domain having at least 97% sequence identity to the sequence of amino acids 100-430 of SEQ ID NO: 4.
29. The process of claim 13 , wherein the polypeptide having endoglucanase activity comprises the sequence of amino acids 100-430 of SEQ ID NO: 4.
30. The process of claim 13 , wherein the composition further comprises a cellobiohydrolase, a beta-glucosidase, and a GH61 polypeptide having cellulolytic enhancing activity.