Stable and selective formation of hoogsteen-type triplexes and duplexes using twisted intercalating nucleic acids (TINA) and process for the preparation of TINA
The present invention describes a flexible basestacking monomer that can be incorporated into an oligonucleotide or oligonucleotide analogue, as well as triplex forming oligonucleotides comprising the flexible basestacking monomer. Triplex forming oligonucleotides of the invention are capable of binding sequence specifically to doublestranded target nucleic acids and are therefore of interest for modulation of the activity of target nucleic acids and also detection of target nucleic acids.
1. An oligonucleotide comprising a flexible basestacking monomer with the structure
X-L-I 1 -C-I 2
wherein:
X is a backbone monomer unit that can be incorporated into the backbone of a oligonucleotide or a oligonucleotide analogue, or PNA, or PNA analogues, wherein X comprises an alkylenediol, ethyleneglycol, or 1-O-methyleneglycerol, which optionally has the alkylenediol partly comprised in a ring system,
L is a linker,
I 1 is a first intercalator comprising at least one essentially flat conjugated system, which is capable of co-stacking with nucleobases of DNA, RNA or analogues thereof, wherein I 1 is a monocyclic or polycyclic aromatic ring system selected from benzene, naphthalene, azulene, or bicyclic heteroaromatic ring systems or substitutions thereof,
C is a conjugator selected from alkyl of 1 to 12 carbons, alkenyl of 2 to 12 carbons, alkynyl of 2 to 25 carbons or diazo or combinations thereof with a length of no more than 25 carbon or nitrogen atoms, and
I 2 is a second intercalator comprising at least one essentially flat conjugated system, which is capable of co-stacking with nucleobases of DNA, RNA or analogues thereof, wherein I 2 is selected from bi-cyclic aromatic ring systems, tricyclic aromatic ring systems, tetracyclic aromatic ring systems, pentacyclic aromatic ring systems or heteroaromatic analogues thereof or substitutions thereof, and
wherein the flexible basestacking monomer leads to high thermal stability of Hoogsteen-type triplexes and duplexes, and
wherein the oligonucleotide comprising a flexible basestacking monomer comprises the structure
2. The oligonucleotide of claim 1 wherein the phenyl ring is ortho-substituted.