Methods for determining and inhibiting rheumatoid arthritis associated with the BRAF oncogene in a subject
The invention provides methods for determining whether a subject is suffering from a rheumatoid arthritis associated with the BRAF oncogene comprising contacting isolated fibroblasts from the subject with a molecule or pool of molecules directed to the BRAF oncogene; and culturing the sample in the presence of the agent and determining whether BRAF oncogene expression by the cell is decreased and/or whether cells in the sample return to a less transformed phenotype, exhibit decreased cell proliferation and/or exhibit increased contact inhibition, any of which is indicative that the subject is suffering from a rheumatoid arthritis associated with the BRAF oncogene.
1. A method of identifying a molecule that binds or blocks a BRAF protein in a synovial fibroblast associated with a BRAF oncogene comprising:
a. contacting a molecule of interest with the BRAF protein, and
b. determining whether the molecule of interest alters BRAF protein activity in the fibroblast, alteration of the BRAF protein activity being indicative that the molecule of interest binds or blocks the BRAF protein in the fibroblast.