Renovation and repopulation of decellularized tissues and cadaveric organs by stem cells
A method of manufacturing a tissue matrix for implantation into a patient is disclosed. The method sets forth collecting embryonic stem cells from a placenta which has been treated to remove residual cord blood and seeding the collected stem cells onto or into a tissue matrix. The seeded tissue matrix is then implanted on or into a patient. The seeded tissue matrix made by the method of the present invention is also disclosed.
1. A method of treating an individual in need of repair of traumatic injury of the skin comprising administering to the individual isolated placental stem cells, wherein said isolated placental stem cells have been seeded onto a decellularized or synthetic tissue matrix, wherein said placental stem cells are obtained from a mammalian placenta that has been drained of cord blood, and wherein said placental stem cells
(a) express antigens recognized by SH2, SH3 and SH4 antibodies;
(b) do not express MHC Class 2 antigens;
(c) are CD34 − , SSEA3 − , SSEA4 − , and OCT-4 + ;
(d) are SSEA3 − , SSEA4 − , OCT-4 + and ABC-p + ; or
(e) are CD10 + , CD29 + , CD34 − , CD44 + , CD45 − , CD54 + , CD90 + , SH2 + , SH3 + , SH4 + , SSEA3 − , SSEA4 − , OCT-4 + and ABC-p + .
2. The method of claim 1 , wherein said placental stem cells express antigens recognized by SH2, SH3 and SH4 antibodies.
3. The method of claim 1 , wherein said placental stem cells do not express MHC Class 2 antigens.
4. The method of claim 1 , wherein said placental stem cells are CD34 − , SSEA3 − , SSEA4 − , and OCT-4 + .
5. The method of claim 1 , wherein said placental stem cells are SSEA3 − , SSEA4 − , OCT-4 + and ABC-p + .
6. The method of claim 1 , wherein said placental stem cells are CD10 + , CD29 + , CD34 − , CD44 + , CD45 − , CD54 + , CD90 + , SH2 + , SH3 + , SH4 + , SSEA3 − , SSEA4 − , OCT-4 + , and ABC-p + .