Wheat with increased resistant starch levels
A series of independent human-induced non-transgenic mutations found at one or more of the SBEII genes of wheat; wheat plants having these mutations in one or more of their SBEII genes; and a method of creating and finding similar and/or additional mutations of SBEII by screening pooled and/or individual wheat plants. The seeds and flour from the wheat plants of the present invention exhibit an increase in amylose and resistant starch without having the inclusion of foreign nucleic acids in their genomes. Additionally, the wheat plants of the present invention exhibit altered SBEII activity without having the inclusion of foreign nucleic acids in their genomes.
1. A wheat plant comprising a null mutation in each allele of an SBEIIa gene of the A, B, and D genomes, wherein the SBEIIa gene of the A genome encodes an SBEIIa polypeptide comprising a tryptophan to a stop mutation at an amino acid position corresponding to amino acid position 436 of SEQ ID NO: 2, wherein said wheat plant produces seed that germinates, and further wherein grain from said wheat plant has an increased amylose level as compared to grain from a wild type wheat plant.
2. The wheat plant of claim 1 , wherein the SBEIIa polypeptide comprises an amino acid sequence having at least 95% identity or similarity to SEQ ID NO: 2.
3. The wheat plant of claim 1 , wherein the SBEIIa gene of the A genome comprises a guanine to adenine mutation at a nucleotide position corresponding to nucleotide position 5267 of SEQ ID NO: 1.
4. The wheat plant of claim 1 , wherein the SBEIIa gene comprises an at least 95% identity or similarity to SEQ ID NO: 1.
5. The wheat plant of claim 1 , wherein the SBEIIa gene comprises at least 97% identity or similarity to SEQ ID NO: 1.
6. A wheat plant comprising a null mutation in each allele of an SBEIIa gene of the A, B, and D genomes, wherein the SBEIIa gene of the B genome encodes an SBEIIa polypeptide comprising a tryptophan to a stop mutation at an amino acid position corresponding to amino acid position 436 of SEQ ID NO: 4, wherein said wheat plant produces seed that germinates, and further wherein grain from said wheat plant has an increased amylose level as compared to grain from a wild type wheat plant.
7. The wheat plant of claim 6 , wherein the SBEIIa polypeptide comprises an amino acid sequence having at least 95% identity or similarity to SEQ ID NO: 4.
8. The wheat plant of claim 6 , wherein the SBEIIa gene of the B genome comprises a guanine to adenine mutation at a nucleotide position corresponding to nucleotide position 5038 of SEQ ID NO: 3.
9. The wheat plant of claim 6 , wherein the SBEIIa gene comprises at least 95% identity or similarity to SEQ ID NO: 3.
10. The wheat plant of claim 6 , wherein the SBEIIa gene comprises at least 97% identity or similarity to SEQ ID NO: 3.
11. A wheat plant comprising a null mutation in each allele of an SBEIIa gene of the A, B, and D genomes, wherein the SBEIIa gene of the D genome encodes an SBEIIa polypeptide comprising a tryptophan to a stop mutation at an amino acid position corresponding to amino acid position 432 of SEQ ID NO: 6, wherein said wheat plant seed that germinates, and further wherein grain from said wheat plant has an increased amylose level as compared to grain from a wild type wheat plant.
12. The wheat plant of claim 11 , wherein the SBEIIa polypeptide comprises an amino acid sequence having at least 95% identity or similarity to SEQ ID NO: 6.
13. The wheat plant of claim 11 , wherein the SBEIIa gene of the D genome comprises a guanine to adenine mutation at a nucleotide position corresponding to nucleotide position 6305 of SEQ ID NO: 5.
14. The wheat plant of claim 11 , wherein the SBEIIa gene comprises at least 95% identity or similarity to SEQ ID NO: 5.
15. The wheat plant of claim 11 , wherein the SBEIIa gene comprises at least 97% identity or similarity to SEQ ID NO: 5.
16. Wheat grain from the wheat plant of claim 1 .
17. Flour comprising a cell of the wheat grain of claim 16 .
18. A food product comprising a cell of the wheat plant of claim 1 .
19. A wheat seed, plant part or progeny thereof from the wheat plant of claim 1 having a null mutation in each allele of an SBEIIa gene of the A, B, and D genomes, wherein the SBEIIa gene of the A genome encodes an SBEIIa polypeptide comprising a tryptophan to a stop mutation at an amino acid position corresponding to amino acid position 436 of SEQ ID NO: 2.
20. Wheat grain from the wheat plant of claim 6 .
21. Flour comprising a cell of the wheat grain of claim 20 .
22. A food product comprising a cell of the wheat plant of claim 6 .
23. A wheat seed, plant part or progeny thereof from the wheat plant of claim 6 having a null mutation in each allele of an SBEIIa gene of the A, B, and D genomes, wherein the SBEIIa gene of the B genome encodes an SBEIIa polypeptide comprising a tryptophan to a stop mutation at an amino acid position corresponding to amino acid position 436 of SEQ ID NO: 4.
24. Wheat grain from the wheat plant of claim 11 .
25. Flour comprising a cell of the wheat grain of claim 24 .
26. A food product comprising a cell of the wheat plant of claim 11 .
27. A wheat seed, plant part or progeny thereof from the wheat plant of claim 11 having a null mutation in each allele of an SBEIIa gene of the A, B, and D genomes, wherein the SBEIIa gene of the D genome encodes an SBEIIa polypeptide comprising a tryptophan to a stop mutation at an amino acid position corresponding to amino acid position 432 of SEQ ID NO: 6.
28. A wheat plant comprising a null mutation in each allele of an SBEIIa gene of the A, B, and D genomes wherein the SBEIIa gene of the A genome encodes an SBEIIa polypeptide comprising a tryptophan to a stop mutation at an amino acid position corresponding to amino acid position 436 of SEQ ID NO: 2; wherein the SBEIIa gene of the B genome encodes an SBEIIa polypeptide comprising a tryptophan to a stop mutation at an amino acid position corresponding to amino acid position 436 of SEQ ID NO: 4; wherein the SBEIIa gene of the D genome encodes an SBEIIa polypeptide comprising a tryptophan to a stop mutation at an amino acid position corresponding to amino acid position 432 of SEQ ID NO: 6, wherein said wheat plant produces seed that germinates and further wherein grain from said wheat plant has an increased amylose level as compared to grain from a wild type wheat plant.
29. Wheat grain from the wheat plant of claim 28 .
30. Flour comprising a cell of the wheat grain of claim 29 .
31. A food product comprising a cell of the wheat plant of claim 28 .
32. A wheat seed, plant part or progeny thereof from the wheat plant of claim 28 having a null mutation in each allele of an SBEIIa gene of the A, B, and D genomes, wherein the SBEIIa gene of the A genome encodes an SBEIIa polypeptide comprising a tryptophan to a stop mutation at an amino acid position corresponding to amino acid position 436 of SEQ ID NO: 2; wherein the SBEIIa gene of the B genome encodes an SBEIIa polypeptide comprising a tryptophan to a stop mutation at an amino acid position corresponding to amino acid position 436 of SEQ ID NO: 4; wherein the SBEIIa gene of the D genome encodes an SBEIIa polypeptide comprising a tryptophan to a stop mutation at an amino acid position corresponding to amino acid position 432 of SEQ ID NO: 6.