Bacterial ribonucleic acid cell wall compositions and methods of making and using them
The present invention relates to novel bacterial and mycobacterial compositions containing RNA and cell walls, and methods for making and using these compositions. These compositions have immune stimulating and anti-cancer activity.
1. A method for treating an animal having cancer comprising:
administering a composition comprising mycobacterial RNA isolated from mycobacterial cells and mycobacterial cell walls isolated from mycobacterial cells, wherein the composition does not contain phenol or Streptomyces griseus protease, and the isolated mycobacterial RNA is in the form of oligoribonucleotides and polyribonucleotides about 2 to about 150 bases in length, and a pharmaceutically acceptable carrier or delivery system, to the animal having cancer in an amount effective to inhibit the growth of cancer cells or induce apoptosis in cancer cells in the animal.
2. A method for stimulating an immune system in an animal comprising:
administering a composition comprising mycobacterial RNA isolated from mycobacterial cells and mycobacterial cell walls isolated from mycobacterial cells, wherein the composition does not contain phenol or Streptomyces griseus protease, and the isolated mycobacterial RNA is in the form of oligoribonucleotides and polyribonucleotides about 2 to about 150 bases in length, and a pharmaceutically acceptable carrier or delivery system, to the animal in an amount effective to stimulate the immune system in the animal.
3. The method of claim 2 , wherein stimulating the immune system in the animal comprises stimulating the differentiation of hematopoietic progenitor(s), increasing the secretion of cytokines or chemokines, increasing the synthesis of hematopoietic growth factors, activating the nucleotide-binding oligomerization domain 2 (NOD2) receptor, or activating the Toll-like receptor 2 (TLR2) receptor.
4. The method of claim 3 , wherein the hematopoietic growth factors are selected from the group consisting of Macrophage Colony Stimulating Factor (M-CSF), Granulocyte-Macrophage Colony Stimulating Factor (GM-CSF), Granulocyte Colony Stimulating Factor (G-CSF), Stromal Cell Derived Factor-1alpha (SDF-1a) and Leukemia Inhibitory Factor (LIF), and combinations thereof.
5. A method of augmenting the efficacy of cancer therapy or immune stimulant therapy in an animal receiving cancer therapy or immune stimulant therapy comprising administering to the animal receiving cancer therapy or immune stimulant therapy a composition comprising mycobacterial RNA isolated from mycobacterial cells and mycobacterial cell walls isolated from mycobacterial cells, wherein the composition does not contain phenol or Streptomyces griseus protease, and the isolated mycobacterial RNA is in the form of oligoribonucleotides and polyribonucleotides about 2 to about 150 bases in length, and a pharmaceutically acceptable carrier or delivery system, in an amount effective to augment the efficacy of cancer therapy or immune stimulant therapy in the animal.
6. The method of claim 1 , wherein the composition further comprises intact mycobacterial cells.
7. The method of claim 2 , wherein the composition further comprises intact mycobacterial cells.
8. The method of claim 5 , wherein the composition further comprises intact mycobacterial cells.
9. The method of claim 6 , wherein the intact mycobacterial cells comprise from about 0.05% to about 95% or about 0.19% to about 19.6% by weight (wt %) of the composition.
10. The method of claim 7 , wherein the intact mycobacterial cells comprise from about 0.05% to about 95% or about 0.19% to about 19.6% by weight (wt %) of the composition.
11. The method of claim 8 , wherein the intact mycobacterial cells comprise from about 0.05% to about 95% or about 0.19% to about 19.6% by weight (wt %) of the composition.
12. The method of claim 1 , wherein the mycobacterial cell is selected from the group consisting of Mycobacterium phlei, Mycobacterium vaccae, Mycobacterium smegmatis, Mycobacterium avium subspecies paratuberculosis and Mycobacterium bovis BCG.
13. The method of claim 2 , wherein the mycobacterial cell is selected from the group consisting of Mycobacterium phlei, Mycobacterium vaccae, Mycobacterium smegmatis, Mycobacterium avium subspecies paratuberculosis and Mycobacterium bovis BCG.
14. The method of claim 5 , wherein the mycobacterial cell is selected from the group consisting of Mycobacterium phlei, Mycobacterium vaccae, Mycobacterium smegmatis, Mycobacterium avium subspecies paratuberculosis and Mycobacterium bovis BCG.
15. The method of claim 12 , wherein the mycobacterial cell is Mycobacterium phlei.
16. The method of claim 13 , wherein the mycobacterial cell is Mycobacterium phlei.
17. The method of claim 14 , wherein the mycobacterial cell is Mycobacterium phlei.
18. The method of claim 1 , wherein the oligoribonucleotides and polyribonucleotides are about 20 to about 40 bases in length, or about 2 to about 40 bases in length.
19. The method of claim 2 , wherein the oligoribonucleotides and polyribonucleotides are about 20 to about 40 bases in length, or about 2 to about 40 bases in length.
20. The method of claim 5 , wherein the oligoribonucleotides and polyribonucleotides are about 20 to about 40 bases in length, or about 2 to about 40 bases in length.
21. The method of claim 1 , wherein the effective amount is from about 0.00001 mg/kg to about 100 mg/kg per dose, from about 0.0001 mg/kg to about 50 mg/kg per dose, from about 0.001 mg/kg to about 10 mg/kg per dose, from about 0.01 mg/kg to about 5 mg/kg per dose, or from about 0.1 mg/kg to about 1 mg/kg per dose.
22. The method of claim 2 , wherein the effective amount is from about 0.00001 mg/kg to about 100 mg/kg per dose, from about 0.0001 mg/kg to about 50 mg/kg per dose, from about 0.001 mg/kg to about 10 mg/kg per dose, from about 0.01 mg/kg to about 5 mg/kg per dose, or from about 0.1 mg/kg to about 1 mg/kg per dose.
23. The method of claim 5 , wherein the effective amount is from about 0.00001 mg/kg to about 100 mg/kg per dose, from about 0.0001 mg/kg to about 50 mg/kg per dose, from about 0.001 mg/kg to about 10 mg/kg per dose, from about 0.01 mg/kg to about 5 mg/kg per dose, or from about 0.1 mg/kg to about 1 mg/kg per dose.
24. The method of claim 1 , wherein the cancer is bladder cancer, colon cancer, leukemia, melanoma, lung cancer, rectal cancer or cervical cancer.