Antitumor peptide derived from a complementarity determining region of a humanized monoclonal antibody to NaPi2B transporter
Described herein is novel isolated or synthetic peptides derived from a complementarity determining region hypervariable domain amino acid sequence of a humanized monoclonal antibody to NaPi2B transporter, as well as derivatives thereof, and a pharmaceutical composition and a method for inhibiting tumor growth or treating a tumor or cancer treating using the antitumor peptides and derivatives thereof.
1. A pharmaceutical composition comprising an antitumor peptide selected from the group consisting of SEQ ID NOS: 3, 8, 9, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, and 33, and a pharmaceutically acceptable carrier or diluent.
2. An isolated or synthetic antitumor peptide according to claim 1 , wherein the peptide of SEQ ID NO:8 is cyclic.
3. A pharmaceutical composition according to claim 1 , wherein the pharmaceutical composition in a form of a tablet, capsule, solution, powder, inhalant, lotion, tincture, troche, suppository, or transdermal patch.
4. A pharmaceutical composition for parenteral, intradermal, or subcutaneous administration, comprising the pharmaceutical composition of claim 1 and a sterile diluent selected from water, saline, glycerin, fixed oils, polyethylene glycols, propylene glycol, or other synthetic solvents; an antibacterial agent selected from benzyl alcohol or methyl parabens; an antioxidant selected from ascorbic acid or sodium bisulfite; a chelating agent; and a buffering agent selected from acetate or phosphate.
5. A pharmaceutical composition for injection or intravenous administration, comprising the pharmaceutical composition of claim 1 and a carrier, wherein the carrier is selected from the group consisting of water, physiological saline, bacteriostatic water, Cremophor EL™ (BASF, Parsippany, N.J.) phosphate buffered saline (PBS), ethanol, polyols, and mixtures thereof;
a coating for maintaining fluidity selected from lecithin or a surfactant;
an antibacterial and antifungal agent selected from parabens, chlorobutanol, phenol, ascorbic acid, and thimerosal;
an agent for maintaining isotonicity selected from sugars, polyalcohols and sodium chloride.
6. A pharmaceutical composition for oral administration, comprising the pharmaceutical composition of claim 1 and an inert diluent or an edible carrier selected from starches or sugars; a binder selected from the group of microcrystalline cellulose, or gelatin; an excipient corresponding to a starch; a sugar selected from the group of lactose, fructose, glucose, methylcellulose, ethylcellulose; a gum selected from gum tragacanth, acacia; a disintegrating agent selected from the group of alginic acid, Primogel, or corn starch; a lubricant selected from magnesium stearate or Sterotes; a glidant selected from colloidal silicon dioxide; a sweetening agent selected from sucrose or saccharin; a flavoring agent selected from the group of peppermint, methyl salicylate, or orange flavoring; and a biodegradable polymer selected from poly-D, L-lactide-co-glycolide or polyglycolide.
7. A pharmaceutical composition for parenteral, intradermal, or subcutaneous administration, comprising the pharmaceutical composition of claim 2 and a sterile diluent selected from water, saline, glycerin, fixed oils, polyethylene glycols, propylene glycol, or other synthetic solvents; an antibacterial agent selected from benzyl alcohol or methyl parabens; an antioxidant selected from ascorbic acid or sodium bisulfite; a chelating agent; and a buffering agent selected from acetate or phosphate.
8. A pharmaceutical composition for injection or intravenous administration, comprising the pharmaceutical composition of claim 2 and a carrier, wherein the carrier is selected from the group consisting of water, physiological saline, bacteriostatic water, Cremophor EL™ (BASF, Parsippany, N.J.) phosphate buffered saline (PBS), ethanol, polyols, and mixtures thereof;
a coating for maintaining fluidity selected from lecithin or a surfactant;
an antibacterial and antifungal agent selected from parabens, chlorobutanol, phenol, ascorbic acid, and thimerosal;
an agent for maintaining isotonicity selected from sugars, polyalcohols and sodium chloride.
9. A pharmaceutical composition for oral administration, comprising the pharmaceutical composition of claim 2 and an inert diluent or an edible carrier selected from starches or sugars; a binder selected from the group of microcrystalline cellulose, or gelatin; an excipient corresponding to a starch; a sugar selected from the group of lactose, fructose, glucose, methylcellulose, ethylcellulose; a gum selected from gum tragacanth, acacia; a disintegrating agent selected from the group of alginic acid, Primogel, or corn starch; a lubricant selected from magnesium stearate or Sterotes; a glidant selected from colloidal silicon dioxide; a sweetening agent selected from sucrose or saccharin; a flavoring agent selected from the group of peppermint, methyl salicylate, or orange flavoring; and a biodegradable polymer selected from poly-D, L-lactide-co-glycolide or polyglycolide.