Human aminoacyl-tRNA synthetase polypeptides useful for the regulation of angiogenesis
Methods of enhancing angiogenesis utilizing carboxy-terminal truncated human tyrosyl-tRNA synthetase (TyrRS) are disclosed. The truncated human TyrRS polypeptides comprise residues 1-364 or residues 1-343 of full length human TyrRS (SEQ ID NO: 2).
1. A method for enhancing angiogenesis in a mammal to achieve a therapeutic effect; the method comprising the step of administering to the mammal an effective amount of a polypeptide which consists essentially of residues 1 to 364 of SEQ ID NO:2 or a dimer thereof encoded by a nucleic acid comprising a coding region consisting of a nucleotide sequence at least 95% identical to a sequence selected from the group consisting of:
(a) a polynucleotide encoding a polypeptide consisting of amino acid residues 1-364 of SEQ ID NO: 2;
(b) a polynucleotide consisting of nucleotide residues 3428-4519 of SEQ ID NO: 1, which encodes amino acid residues 1-364 of SEQ ID NO: 2; and
(c) a polynucleotide that is hybridizable to a polynucleotide consisting of nucleotide residues 3428-4519 of SEQ ID NO: 1, which encodes amino acid residues 1-364 of SEQ ID NO: 2.
2. The method of claim 1 wherein the polypeptide consists essentially of residues 1 to 364 of SEQ ID NO:2 or a dimer thereof.
3. The method of claim 1 wherein the therapeutic effect comprises enhancing angiogenesis in a tissue graft in the mammal.
4. A method of treating a condition that would benefit from increased angiogenesis in a mammal comprising the step of administering to the mammal an angiogenically effective amount of a polypeptide which consists essentially of residues 1 to 364 of SEQ ID NO:2 or a dimer thereof encoded by a nucleic acid comprising a coding region consisting of a nucleotide sequence at least 95 % identical to a sequence selected from the group consisting of:
(a) a polynucleotide encoding a polypeptide consisting of amino acid residues 1-364 of SEQ ID NO: 2;
(b) a polynucleotide consisting of nucleotide residues 3428-4519 of SEQ ID NO: 1, which encodes amino acid residues 1-364 of SEQ ID NO: 2; and
(c) a polynucleotide that is hybridizable to a polynucleotide consisting of nucleotide residues 3428-4519 of SEQ ID NO: 1, which encodes amino acid residues 1-364 of SEQ ID NO: 2.
5. The method of claim 4 wherein the polypeptide consists essentially of residues 1 to 364 of SEQ ID NO:2 or a dimer thereof.
6. The method of claim 4 wherein the condition that would benefit from increased angiogenesis comprises a myocardial infarction.