Antibodies against clostridium difficile toxins and uses thereof
Antibodies that specifically bind to toxins of C. difficile , antigen binding portions thereof, and methods of making and using the antibodies and antigen binding portions thereof are provided herein.
1. An isolated nucleic acid comprising a nucleotide sequence encoding a polypeptide, wherein the polypeptide comprises SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:5, SEQ ID NO:6, SEQ ID NO:54, or SEQ ID NO:58.
2. An isolated nucleic acid comprising a nucleotide sequence encoding an antibody variable region, wherein the antibody variable region is at least 90% identical to SEQ ID NO:1, at least 90% identical to SEQ ID NO:2, at least 96% identical to SEQ ID NO:3, at least 99% identical to SEQ ID NO:5, at least 99% identical to SEQ ID NO:6, or at least 95% identical to SEQ ID NO:54.
3. The isolated nucleic acid of claim 2 , wherein the antibody variable region is at least 90% identical to SEQ ID NO:1.
4. The isolated nucleic acid of claim 2 , wherein the antibody variable region is at least 90% identical to SEQ ID NO:2.
5. The isolated nucleic acid of claim 2 , wherein the antibody variable region is at least 96% identical to SEQ ID NO:3.
6. The isolated nucleic acid of claim 1 , wherein the polypeptide comprises SEQ ID NO:4.
7. The isolated nucleic acid of claim 2 , wherein the antibody variable region at least 99% identical to SEQ ID NO:5.
8. The isolated nucleic acid of claim 2 , wherein the antibody variable region is at least 99% identical to SEQ ID NO:6.
9. The isolated nucleic acid of claim 2 , wherein the antibody variable region is at least 95% identical to SEQ ID NO:54.
10. An expression vector comprising the nucleic acid of claim 2 .
11. A host cell comprising the nucleic acid of claim 2 .
12. The host cell of claim 11 , wherein the host cell is selected from the group consisting of a bacterial cell, a eukaryotic cell, or and a mammalian cell.
13. An isolated nucleic acid comprising a nucleotide sequence set forth in SEQ ID NO:38, SEQ ID NO:35, SEQ ID NO:39, SEQ ID NO:36, SEQ ID NO:40, SEQ ID NO:37, SEQ ID NO:55, or SEQ ID NO:59.
14. The isolated nucleic acid of claim 13 comprising the nucleotide sequence set forth in SEQ ID NO:38.
15. The isolated nucleic acid of claim 13 comprising the nucleotide sequence set forth in SEQ ID NO:35.
16. The isolated nucleic acid of claim 13 comprising the nucleotide sequence set forth in SEQ ID NO:39.
17. The isolated nucleic acid of claim 13 comprising the nucleotide sequence set forth in SEQ ID NO:36.
18. The isolated nucleic acid of claim 13 comprising the nucleotide sequence set forth in SEQ ID NO:40.
19. The isolated nucleic acid of claim 13 comprising the nucleotide sequence set forth in SEQ ID NO:37.
20. The isolated nucleic acid of claim 13 comprising the nucleotide sequence set forth in SEQ ID NO:55.
21. The isolated nucleic acid of claim 13 comprising the nucleotide sequence set forth in SEQ ID NO:59.
22. An expression vector comprising the nucleic acid of claim 13 .
23. A host cell comprising the nucleic acid of claim 13 .
24. The host cell of claim 23 , wherein the host cell is selected from the group consisting of a bacterial cell, a eukaryotic cell, and a mammalian cell.
25. The isolated nucleic acid of claim 1 , wherein the polypeptide comprises SEQ ID NO:58.