IP Library › Granted Patent US 9,217,154
Granted Patent B2
US 9,217,154 · App. 14/363,810 · Granted Dec 22, 2015

Actinomycete integrative and conjugative element from

Inventors: Andreas Klein (Nordkirchen, DE); Klaus Selber (Haan, DE); Hermann Wehlmann (Wuppertal, DE); Winfried Rosen (Remscheid, DE); Alfred Pühler (Bielefeld, DE); Patrick Schwientek (Davis, CA); Jörn Kalinowski (Bielefeld, DE); Udo Wehmeier (Wuppertal, DE)
Assignee: BAYER INTELLECTUAL PROPERTY GMBH
C12N15/74C07K14/365C12N15/52C12N15/76C12P19/26C12P19/28
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Quick Facts
Patent No.
US 9,217,154
App. No.
14/363,810
Granted
Dec 22, 2015
Kind
B2
Abstract

The present invention is directed to an innate DNA sequence within the complete genome sequence of Actinoplanes sp. SE50/110 which resembles the structure of an actinomycete integrative and conjugative element (AICE). Related AICEs were used for establishing genetic manipulation tools for other bacteria in the past. In this document, we describe the unique features of the specific AICE found in Actinoplanes sp. SE50/110 which are clearly distinct from any other known AICE as a whole, but share minor parts with varying sequence similarity with other characterized AiCEs from other species.

Claims (7)

1. A host cell transformed with a heterologous polynucleotide having the sequence of SEQ ID NO:1.

2. The host cell of claim 1 wherein the host cell is an Actinoplanes sp.

3. A method for preparation of biological products comprising the steps of

culturing the host cell of claim 1 in a useful medium,

harvesting the product from the culture and

isolating and purifying the product.

4. The method of claim 3 wherein the product is acarbose.

Assignments (1)
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Jul 7, 2014
From: KLEIN, ANDREAS, DR.; SELBER, KLAUS, DR.; WEHLMANN, HERMANN, DR.; ROSEN, WINFRIED, DR.; PUEHLER, ALFRED, DR.; SCHWIENTEK, PATRICK; KALINOWSKI, JÖRN, DR.; WEHMEIER, UDO, DR.
To: BAYER INTELLECTUAL PROPERTY GMBH
Reel/Frame 033250/0167 →
Priority Claims (1)
EP 11192618 · Dec 8, 2011 · regional
Continuity (1)
Related Publication 20140349346A1 · Nov 27, 2014