IP Library Granted Patent US 9,222,821
Granted Patent B2
US 9,222,821 · App. 13/776,101 · Granted Dec 29, 2015

Volumetric measurement

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Quick Facts
Patent No.
US 9,222,821
App. No.
13/776,101
Granted
Dec 29, 2015
Kind
B2
Abstract

Disclosed is a method of determining a volume of liquid in a sample tube, comprising the steps of capturing an image of the sample tube, determining a first region of interest within the sample tube based upon pre-stored information concerning dimensional properties of the sample tube, scanning the first region of interest to detect the position of a meniscus indicative of an upper extent of the liquid, and using said meniscus position together with certain pre-stored properties of the sample tube to determine a volume of liquid in the tube, and outputting said volume. Also disclosed is an apparatus for performing the method.

Claims (35)

1. A method of determining a volume of liquid in a sample tube, comprising the steps of:

capturing an image of the sample tube;

determining a first region of interest within the sample tube based upon the image and pre-stored information concerning dimensional properties of the sample tube;

scanning the first region of interest to detect the position of a meniscus indicative of an upper extent of the liquid;

determining a volume of liquid in the tube by using said meniscus position as determined from the image together with certain pre-stored properties of the sample tube; and

outputting said volume.

2. The method of claim 1 wherein the step of scanning the first region of interest comprises the use of an edge-detect algorithm to locate a discontinuity within the first region of interest.

3. The method of claim 1 wherein the step of determining the volume comprises inputting the measured meniscus position to a table of pre-stored data to determine a corresponding volume.

4. The method of claim 1 wherein the step of outputting said volume comprises outputting to a database for further analysis.

5. The method of claim 1 wherein the step of outputting said volume comprises outputting via a display device to a user.

6. The method of claim 1 comprising the further steps of:

defining a second region of interest, positioned below the meniscus position;

scanning said second region of interest to detect a possible discontinuity in the second region of interest; and

recording the presence or absence of a precipitate depending upon the presence or absence of a discontinuity.

7. The method of claim 1 comprising the further steps of detecting the presence or absence of a cap on the sample tube and recording the cap presence or absence result.

8. The method of claim 1 comprising the further steps of reading a bar code associated with one or more sample tubes and recording the result.

9. The method of claim 1 wherein the step of capturing an image of the sample tube includes capturing the image of a plurality of sample tubes simultaneously.

10. An apparatus operable to determine a volume of liquid in a sample tube, comprising:

a camera for capturing an image of the sample tube; and

an image processor operable to

determine a first region of interest within the sample tube based upon the image and pre-stored information concerning dimensional properties of the sample tube,

scan the first region of interest to detect the position of a meniscus, indicative of an upper extent of the liquid,

determine a volume of liquid in the tube by using said meniscus position as determined from the image together with certain pre-stored properties of the sample tube, and

output said volume.

11. The apparatus of claim 10 wherein the image processor is operable to scan the first region of interest, and to use an edge-detect algorithm to locate a discontinuity within the first region of interest.

12. The apparatus of claim 10 wherein the image processor is operable to input the measured meniscus position to a table of pre-stored data to determine a corresponding volume.

13. The apparatus of claim 10 wherein the image processor is operable to output to a database for further analysis.

14. The apparatus of claim 10 wherein the image processor is operable to output via a display device to a user.

15. The apparatus of claim 10 wherein the image processor is operable to

define a second region of interest positioned below the meniscus position,

scan said second region of interest to detect a possible discontinuity in the second region of interest, and

record the presence or absence of a precipitate depending upon the presence or absence of a discontinuity.

16. The apparatus of claim 10 wherein the image processor is operable to detect the presence or absence of a cap on the sample tube, and to record the cap presence or absence result.

17. The apparatus of claim 10 further comprising a bar code reader for reading a bar code associated with one or more sample tubes.

18. The apparatus of claim 10 operable to capture a plurality of sample tube images simultaneously.

Assignments (3)
RELEASE OF SECURITY INTEREST RECORDED AT REEL/FRAME 044142/0258 Recorded Nov 4, 2025
From: MORGAN STANLEY SENIOR FUNDING, INC.
To: AZENTA, INC. (F/K/A BROOKS AUTOMATION, INC.); AZENTA US, INC. (F/K/A BROOKS LIFE SCIENCES, INC., F/K/A BIOSTORAGE TECHNOLOGIES, INC.)
Reel/Frame 073514/0609 →
SECURITY INTEREST Recorded Oct 6, 2017
From: BROOKS AUTOMATION, INC.; BIOSTORAGE TECHNOLOGIES, INC.
To: MORGAN STANLEY SENIOR FUNDING, INC.
Reel/Frame 044142/0258 →
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Jul 11, 2013
From: WALSH, CHRISTOPHER; LEE, DAVID CHARLES; SHORT, MARTIN GEOFFREY
To: BROOKS AUTOMATION, INC.
Reel/Frame 030779/0434 →