Methods and compositions for oxidizing bisphenol A
Disclosed are methods, compositions and kits pertaining to polymer coatings that entrap enzymes, specifically enzymes capable of degrading bisphenol A.
1. A composition comprising:
a bisphenol A (BPA)-containing substance; and
at least one BPA-degrading layer coated onto the BPA-containing substance, wherein the BPA-degrading layer comprises a sol and a plurality of polymer particles embedded in the sol, wherein BPA-degrading enzymes are entrapped in the polymer particles, and wherein the polymer particles comprise an organic silane formed from polymerization of methyltrimethoxysilane and gamma-aminopropyltriethoxysilane.
2. The composition of claim 1 , wherein the BPA-containing substance comprises a polycarbonate plastic or an epoxy resin.
3. The composition of claim 1 , wherein the BPA-degrading enzyme oxidizes BPA.
4. The composition of claim 1 , wherein the BPA-degrading enzyme is one or more selected from the group consisting of a potato enzyme extract, a laccase, a tyrosinase, or a peroxidase.
5. The composition of claim 1 , wherein the BPA-degrading enzyme comprises a glucuronosyltransferase.
6. The composition of claim 1 , wherein the BPA-degrading enzyme comprises a sulfotransferase.
7. A method of treating a bisphenol A (BPA)-containing substance, the method comprising:
applying a BPA-degrading coating to a surface of a BPA-containing substance, wherein the BPA-degrading coating comprises a sol and a plurality of polymer particles embedded in the sol, wherein BPA-degrading enzymes are entrapped in the polymer particles, and wherein the polymer particles comprise an organic silane formed from polymerization of methyltrimethoxysilane and gamma-aminopropyltriethoxysilane.
8. The method of claim 7 , wherein applying the BPA-degrading coating comprises applying a BPA-degrading coating comprising BPA-degrading enzymes entrapped in polymer particles, wherein the polymer particles form a gel suspended in an adhesive film.
9. The method of claim 7 , wherein applying the BPA-degrading coating to the surface of the BPA-containing substance comprises applying a BPA-degrading coating to a BPA-containing substance selected from a polycarbonate plastic and/or an epoxy resin.
10. The method of claim 7 , wherein applying the BPA-degrading coating to the surface of the BPA-containing substance comprises applying a BPA-degrading coating comprising a BPA-degrading enzyme that oxidizes BPA.
11. The method of claim 7 , wherein applying the BPA-degrading coating to the surface of the BPA-containing substance comprises applying a BPA-degrading coating comprising at least one BPA-degrading enzyme selected from a potato enzyme extract, a laccase, a tyrosinase, or a peroxidase.
12. The method of claim 7 , wherein applying the BPA-degrading coating to the surface of the BPA-containing substance comprises applying a BPA-degrading coating comprising a glucuronosyltransferase.
13. The method of claim 7 , wherein applying the BPA-degrading coating to the surface of the BPA-containing substance comprises applying a BPA-degrading coating comprising a sulfotransferase.
14. A method of reducing release of bisphenol A (BPA) from a BPA-containing substance comprising:
applying a BPA-degrading coating to a surface of a BPA-containing substance, wherein the BPA-degrading coating comprises a sol and a plurality of polymer particles embedded in the sol, wherein BPA-degrading enzymes are entrapped in the polymer particles, and wherein the polymer particles comprise an organic silane formed from polymerization of methyltrimethoxysilane and gamma-aminopropyltriethoxysilane.
15. The method of claim 14 , wherein applying the BPA-degrading coating comprises applying a BPA-degrading coating comprising BPA-degrading enzymes entrapped in polymer particles, wherein the polymer particles form a gel suspended in an adhesive film.
16. The method of claim 14 , wherein applying the BPA-degrading coating to the surface of the BPA-containing substance comprises applying a BPA-degrading coating to a BPA-containing substance selected from a polycarbonate plastic and/or an epoxy resin.
17. The method of claim 14 , wherein applying the BPA-degrading coating to the surface of the BPA-contain substance comprises applying a BPA-degrading coating comprising a BPA-degrading enzyme that oxidizes BPA.
18. The method of claim 14 , wherein applying the BPA-degrading coating to the surface of the BPA-contain substance comprises applying a BPA-degrading coating comprising at least one BPA-degrading enzyme selected from a potato enzyme extract, a laccase, a tyrosinase, or a peroxidase.
19. The method of claim 14 , wherein applying the BPA-degrading coating to the surface of the BPA-containing substance comprises applying a BPA-degrading coating comprising a glucuronosyltransferase.
20. The method of claim 14 , wherein applying the BPA-degrading coating to the surface of the BPA-containing substance comprises applying a BPA-degrading coating comprising a sulfotransferase.
21. The composition of claim 1 , wherein the sol comprises one or more sols selected from the group consisting of sol of titanium oxide, sol of methoxytrimethylsilane, and sol of dimethyldimethoxysilane.
22. The composition of claim 1 , wherein the polymer particles comprise nanoparticles.