Amino acid sequence presenting fusion polypeptide and its use
Herein is reported a fusion polypeptide according to formula (I): NH 2 —S 2 —X 1 —S 1 —COOH, wherein X 1 comprises either a random amino acid sequence or an amino acid sequence derived from a first polypeptide, S2 and S1 are non-overlapping amino acid sequences derived from a second polypeptide, and denotes a peptide bond, wherein the second polypeptide is a polypeptide with peptidyl-prolyl cis/trans-isomerase activity (PPIase activity) or is derived from the FKBP-fold domain family, wherein X 1 is inserted in place of the insert-in-flap-domain of the second polypeptide.
1. A method for eliciting an immune response in an experimental animal against a polypeptide said method comprising: administering to the experimental animal a fusion polypeptide for at least one time, said fusion polypeptide comprising a polypeptide according to formula I:
NH 2 —S 2 —X 1 —S 1 —COOH (formula I),
wherein:
X 1 comprises a random amino acid sequence or an amino acid sequence derived from a first polypeptide,
S 2 and S 1 are non-overlapping amino acid sequences derived from a second polypeptide, and
denotes a peptide bond,
wherein the second polypeptide is selected from the group consisting of human FKBP12, Arabidopsis thaliana FKBP13, Thermus thermophilus SlyD, Escherichia coli SlyD and Thermococcus gammatolerans SlyD, and
wherein X 1 is inserted in place of the insert-in-flap-domain (IF-domain) of the second polypeptide, and
wherein X 1 is the amino acid sequence of the polypeptide.