Systems and user interface for collecting a data set in a flow cytometer
Systems in a flow cytometer having an interrogation zone and illumination impinging the interrogation zone include: a lens subsystem including a collimating element that collimates light from the interrogation zone, a light dispersion element that disperses collimated light into a light spectrum, and a focusing lens that focuses the light spectrum onto an array of adjacent detection points; a detector array, including semiconductor detector devices, that collectively detects a full spectral range of input light signals, in which each detector device detects a subset spectral range of the full spectral range of light signals; and a user interface that enables a user to create a set of virtual detector channels by grouping detectors in the detector array, such that each virtual detector channel corresponds to a detector group and has a virtual detector channel range including the sum of subset spectral ranges of the detectors in the corresponding detector group.
1. A system for collecting a data set for a flow cytometer sample in a flow cytometer, comprising:
a detector array, including a plurality of detectors, that collectively detects a full spectral range of a set of input signals from the flow cytometer, wherein each detector detects a subset spectral range of the full spectral range, and wherein subset spectral ranges of the plurality of detectors overlap; and
a user interface that enables a user to create a set of virtual detector channels by grouping detectors in the detector array, such that each virtual detector channel corresponds to a detector group and corresponds to a grouping of input signals from the set of input signals, wherein the input signals are detected by the detectors in the corresponding detector group.
2. The system of claim 1 , wherein the user interface enables the user to configure, from the plurality of detectors, a first set of detector groups corresponding to a first set of virtual detector channels, and to configure, from the plurality of detectors, a second set of detector groups corresponding to a second set of virtual detector channels, wherein the first and second sets of detector groups are different.
3. The system of claim 2 , wherein the detector array collectively detects a wide range of input signals that includes at least a 1:1,000,000 ratio between the faintest objects and the brightest objects.
4. The system of claim 1 , wherein the detector array is a linear array of detectors.
5. The system of claim 4 , wherein the linear array of detectors includes a plurality of photodiodes.
6. The system of claim 4 , wherein the linear array of detectors includes a plurality of phototransistors.
7. The system of claim 1 , wherein the subset spectral ranges of the plurality of detectors are contiguous.
8. The system of claim 1 , wherein the detector array detects a full spectral range of input signals of approximately 400 nm to 900 nm.
9. The system of claim 8 , wherein the subset spectral ranges of the plurality of detectors are of approximately equal span.
10. A method for collecting a data set for a flow cytometer sample in a flow cytometer, comprising the steps of:
providing a detector array having a plurality of detectors that collectively detect a full spectral range of a set of input signals from the flow cytometer, wherein each detector detects a subset spectral range of the full spectral range, and wherein subset spectral ranges of the plurality of detectors overlap;
creating a set of virtual detector channels by grouping detectors in the detector array, wherein each virtual detector channel corresponds to a detector group and corresponds to a grouping of input signals from the set of input signals, wherein the input signals are detected by the detectors in the corresponding detector group; and
collecting the full spectral range of input signals from the flow cytometer sample with the detector array.
11. The method of claim 10 , wherein collecting input signals includes collecting a wide range of input signals that includes at least a 1:1,000,000 ratio between the faintest objects and the brightest objects.
12. The method of claim 10 , wherein creating a set of virtual detector channels includes receiving a user selection of detector groups.
13. The method of claim 10 , wherein creating a set of virtual detector channels includes receiving a user selection of virtual detector channel ranges and grouping detectors into detector groups that correspond to the selection of virtual detector channel ranges.
14. The method of claim 10 , wherein creating a set of virtual detector channels includes receiving at least one configuration parameter and optimally grouping detectors based on the at least one configuration parameter.
15. The method of claim 14 , wherein optimally grouping detectors based on the configuration parameter includes at least one of: minimizing spillover between virtual detector channels and maximizing sensitivity of the virtual detector channels.
16. The method of claim 14 , wherein creating a set of virtual detector channels includes receiving a user selection of a set of fluorochromes and assigning a virtual detector channel for each of the selected fluorochromes.
17. The method of claim 10 , wherein creating a set of virtual detector channels includes receiving a configuration file specifying virtual detector channel settings.
18. The method of claim 10 , wherein creating a set of virtual detector channels is performed before collecting input signals.
19. The method of claim 10 , wherein creating a set of virtual detector channels may be performed after collecting input signals.
20. The method of claim 19 , further comprising storing the collected input signals.
21. The method of claim 20 , wherein storing the collected input signals includes storing the input signals as that individually collected by each separate detector in the detector array.
22. The method of claim 20 , wherein storing the collected input signals includes storing the input signals as that collected by each separate virtual detector channel.
23. The method of claim 10 , further comprising allowing a user to configure, from the plurality of detectors, a first set of detector group corresponding to a first set of virtual detector channels, and to configure, from the plurality of detectors, a second set of detector groups corresponding to a second set of virtual detector channels, wherein the first and second sets of detector groups are different.
24. A system for collecting a data set for a flow cytometer sample in a flow cytometer, comprising:
a detector array, including a plurality of detectors, that collectively detects a full spectral range of a set of input signals from the flow cytometer, wherein each detector detects a subset spectral range of the full spectral range, and wherein subset spectral ranges of the plurality of detectors overlap; and
a user interface that enables a user to submit a configuration parameter and create a set of virtual detector channels by grouping detectors in the detector array into a set of detector groups based on the configuration parameter, such that the set of virtual detector channels corresponds to the set of detector groups in a one-to-one manner,
wherein a virtual detector channel of the set of virtual detector channels comprises an aggregation of a subset of input signals from the set of input signals, wherein the subset of input signals is detected by detectors in a detector group corresponding to the virtual detector channel.