Patent
A composition and method for producing plants with increased biomass and growth characteristics, and plants which flower early relative to wild-type plants. The MtNIP gene is over-expressed in plants to increase biomass, growth, and early flowering. Other genes in the NRT1/PTR family may also be over-expressed to increase biomass, growth, and early flowering.
1. A transgenic plant, or parts thereof, with increased biomass compared to a non-transformed plant of the same variety, or parts thereof wherein substantially all cells of the plant have been transformed with a construct comprising a heterologous, constitutive promoter operably linked to a nucleic acid encoding a protein comprising SEQ ID NO:8.
2. A seed of the transgenic plant, or parts thereof, of claim 1 , wherein the seed comprises the construct.
3. The transgenic plant, or parts thereof, of claim 1 , wherein the transgenic plant is a transformed Arabidopsis thaliana and wherein the parts comprise the construct.
4. A seed of the transgenic plant, or parts thereof, of claim 3 , wherein the seed comprises the construct.
5. The transgenic plant, or parts thereof, of claim 1 , wherein the transgenic plant is a transformed Medicago truncatula and wherein the parts comprise the construct.
6. A seed of the transgenic plant, or parts thereof, of claim 5 , wherein the seed comprises the construct.
7. The transgenic plant, or parts thereof, of claim 1 , wherein the transgenic plant is a transformed tobacco plant and wherein the parts comprise the construct.
8. A seed of the transgenic plant, or parts thereof, of claim 7 , wherein the seed or parts comprise the construct.
9. A method of producing a transgenic plant, said method comprising: transforming substantially all cells in a plant with a construct comprising a heterologous, constitutive promoter operably linked to a nucleic acid encoding a protein comprising SEQ ID NO:8.
10. The transgenic plant produced by the method of claim 9 .
11. The method of claim 9 , wherein the plant is Arabidopsis thaliana.
12. A progeny of the transgenic plant produced by the method of claim 9 , wherein the progeny comprises the construct.
13. The method of claim 9 , wherein the plant is M. truncatula.
14. The method of claim 9 , wherein the plant is a tobacco plant.
15. A method for generating transgenic seeds for plants having an increased biomass, comprising the step of:
(a) multiplying plant cells that contain at least one genetic transformation event of interest and which are capable of regeneration; wherein the genetic transformation event comprises a promoter nucleotide sequence that is capable of initiating transcription in a constitutive manner of an operably linked heterologous nucleic acid sequence comprising a nucleic acid sequence encoding a protein having a sequence of SEQ ID NO: 8 into said plant cells;
(b) selecting the plant cells that comprise at least one genetic transformation event of interest;
(c) regenerating a whole transgenic plant, or parts thereof, termed primary transformants, or T 0 plants, from said plant cells;
(d) pollinating said primary transformants with non-transgenic pollen;
(e) harvesting the seeds obtained, termed T 1 , which have integrated at least one transgene of interest;
(f) sowing said transgenic T 1 seeds and pollinating the plants which result therefrom, either by self-pollination or by free pollination; and
(g) harvesting the T 2 seeds.
16. The method of claim 15 , wherein the plant cells are Arabidopsis thaliana plant cells, Medicago truncatula plant cells or tobacco plant cells.
17. An isolated transformation vector construct having a heterologous, constitutive promoter region operably linked to an expression region having at least 95% homology with SeqID No.: 23, SeqID No.: 24, or SeqID No.: 25.
18. A transgenic plant, or parts thereof, comprising a construct comprising a heterologous, constitutive promoter operably linked to a nucleic acid encoding a protein comprising SEQ ID NO:2 or SEQ ID NO:8, wherein the parts comprise the construct.
19. The transgenic plant, or parts thereof, of claim 18 , wherein the plant cells are Arabidopsis thaliana plant cells or a M. truncatula plant cells.
20. The transgenic plant, or parts thereof, of claim 18 , wherein the plant cells are tobacco plant cells.
21. A seed of the transgenic plant of claim 18 , wherein the seed comprises the construct.