Optical device for transmission-type scanning by moving scanning beam without moving observation sample
For an optical device as a transmission-type scanning optical microscope having a pinhole or a slit for limiting the amount of a detected light beam, a method of moving a scanning beam without moving an observation sample to be scanned is realized. A scanning beam from a beam scanning mechanism that has passed through an observation sample is focused onto a reflection plate, and is then returned back again to the observation sample. A light beam that has returned back from the sample is further fed back to the beam scanning mechanism, and then, the light beam that has been limited through a fixed pinhole or a slit is detected with a photodetector.
1. An optical device comprising:
a laser source;
a beam scanning mechanism configured to move a scanning laser beam emitted from the laser source;
a stage configured to hold an observation sample;
an objective lens configured to focus the laser beam output from the beam scanning mechanism onto the observation sample held on the stage;
condenser optics provided on a light-transmission side of the stage;
a reflection plate;
a condenser lens configured to focus a light beam returning back from the beam scanning mechanism;
an aperture arranged at a focal position of the condenser lens;
a photodetector configured to detect a light beam that has passed through the aperture;
an electronic device configured to store a signal of the photodetector in association with a scanning position; and
a display device configured to display the signal stored in the electronic device;
optics configured to detect a part of the light beam returning back from the beam scanning mechanism to detect a focus error;
an actuator configured to drive a lens of the condenser optics or the reflection plate in an optical-axis direction using a signal of the detected focus error
another scanning mechanism including another actuator configured to move another lens arranged between the objective lens and the laser source in the optical-axis direction,
wherein the electronic device generates instructions based on the detected focus error and sends the instructions to the actuator and to the another actuator to position the reflection plate at a focal position of the condenser optics.
2. The optical device according to claim 1 , wherein the aperture is one of a pinhole or a slit.
3. The optical device according to claim 1 , wherein the beam scanning mechanism is a two-dimensional scanning mechanism.
4. The optical device according to claim 1 , wherein the beam scanning mechanism is a three-dimensional scanning mechanism.
5. The optical device according to claim 1 , wherein a slit is used as the aperture, and a spectrometer is used as the photodetector.
6. The optical device according to claim 5 , wherein the light beam returning back from the beam scanning mechanism includes a light beam with a different wavelength from a wavelength of an incident light beam.
7. The optical device according to claim 6 , further comprising:
an optical element configured to generate a supercontinuum beam using the laser beam emitted from the laser source as a pump beam; and
optics configured to detect a part of the light beam returning back from the beam scanning mechanism to detect a focus error;
wherein the observation sample is irradiated with the pump beam and a Stokes beam that is a part of the supercontinuum beam,
wherein an anti-Stokes beam from the observation sample is analysed spectroscopically, and
wherein the optics configured to detect a focus error obtain a focus error signal from the pump beam or the Stokes beam contained in the light beam returning back from the beam scanning mechanism.
8. The optical device according to claim 7 , wherein a part of the supercontinuum beam has a frequency spectrum in a high-frequency region for use as a reference beam.
9. The optical device according to claim 6 , further comprising:
an optical element configured to generate a supercontinuum beam using the laser beam emitted from the laser source as a pump beam; and
a photonic crystal fiber configured to detect a part of the light beam returning back from the beam scanning mechanism to detect a focus error;
wherein the observation sample is irradiated with the pump beam and a Stokes beam that is a part of the supercontinuum beam,
wherein an anti-Stokes beam from the observation sample is analyzed spectroscopically, and
wherein the optics configured to detect a focus error obtain a focus error signal from the pump beam or the Stokes beam contained in the light beam returning back from the beam scanning mechanism.
10. The optical device according to claim 9 , wherein the photonic crystal fiber is a polarization-preserving photonic crystal fiber.