IP Library Granted Patent US 9,302,924
Granted Patent B1
US 9,302,924 · App. 14/633,664 · Granted Apr 5, 2016

Method for reducing cyanuric acid in recreational water systems

Inventors: Michael S. Showell (Cincinnati, OH); Richard S. Carpenter (West Chester, OH); John Gorsuch (Cincinnati, OH); Joseph Roberts (Cincinnati, OH); David Bartoli (Murrieta, CA); Richard Rosene (Murrieta, CA); Lora Rosene (Murrieta, CA)
Assignee: BiOWiSH Technologies, Inc.
C02F3/341C02F1/70C02F2101/38C02F2103/42C02F2305/06C12N1/20
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Quick Facts
Patent No.
US 9,302,924
App. No.
14/633,664
Granted
Apr 5, 2016
Kind
B1
Abstract

The present invention provides compositions and methods of reducing cyanuric acid levels in recreational water systems.

Claims (25)

1. A composition comprising

a. between 75-95% w/w of anhydrous dextrose or dextrose monohydrate;

b. a mixture of Bacillus bacterial species comprising Bacillus subtilis, Bacillus licheniformis, Bacillus amyloliquefaciens , and Bacillus pumilus having a bacterial concentration of at least 1×10 6 colony forming units (CFU) per gram of the mixture, wherein each of the Bacillus species are individually fermented aerobically, dried and ground to an average particle size of about 200 microns, and wherein the Bacillus subtilis comprises Mojavensis;

c. a mixture of Lactobacillus bacterial species comprising Pediococcus acidilactici, Pediococcus pentosaceus , and Lactobacillus plantarum having a bacterial concentration of at least 1×10 6 colony forming units (CFU) per gram of the mixture, wherein each of the Lactobacillus species are fermented anaerobically, dried, and ground to an average particle size of about 200 microns.

2. The composition of claim 1 , wherein the bacterial species are non-pathogenic.

3. The composition of claim 1 , wherein the Bacillus subtilis includes Bacillus subtilis 34 KLB.

4. The composition of claim 1 , wherein each of the Lactobacillus bacterial species are present in equal amounts by weight.

5. The composition according to claim 1 , wherein the composition further comprises an inorganic mineral that stimulates bacterial respiration and growth.

6. The composition according to claim 5 , wherein the inorganic mineral is disodium hydrogen phosphate, dipotassium hydrogen phosphate, sodium dihydrogen phosphate, potassium dihydrogen phosphate, sodium chloride, potassium chloride, magnesium sulfate, calcium sulfate, magnesium chloride, calcium chloride, or iron(III) chloride.

7. A method for reducing cyanuric acid concentration on recreational water systems comprising contacting a pool's filtration system with the composition of claim 1 .

8. A composition comprising

a. between 75-95% w/w of anhydrous dextrose or dextrose monohydrate;

b. at least 1% w/w of a mixture containing Bacillus bacterial species comprising Bacillus subtilis, Bacillus licheniformis, Bacillus amyloliquefaciens , and Bacillus pumilus , wherein each of the Bacillus species are individually fermented aerobically, dried and ground to an average particle size of about 200 microns, and wherein the Bacillus subtilis comprises Mojavensis;

c. at least 4% w/w of a mixture containing Lactobacillus bacterial species comprising Pediococcus acidilactici, Pediococcus pentosaceus , and Lactobacillus plantarum , wherein each of the Lactobacillus species are fermented anaerobically, dried, and ground to an average particle size of about 200 microns.

9. The composition of claim 8 , wherein a bacterial concentration of the Bacillus mixture is at least 1×10 6 colony forming units (CFU) per gram of the mixture and the bacterial concentration the Lactobacillus mixture is at least 1×10 6 colony forming units (CFU) per gram of the mixture.

10. The composition of claim 9 , wherein at least 15% of the Bacillus CFUs are Bacillus subtilis 34 KLB.

11. A composition comprising:

a. at least 0.4% w/w/ of a first Bacillus mixture comprising Bacillus subtilis subsp mojavensis, Bacillus licheniformis, Bacillus amyloliquefaciens , and Bacillus pumilus;

b. at least 0.4% w/w/ of a second Bacillus mixture comprising Bacillus subtilis, Bacillus licheniformis, Bacillus amyloliquefaciens , and Bacillus pumilus;

c. at least 0.4% w/w/ of a mixture o Bacillus subtilis 34 KLB; and

d. at least 4% w/w of a Lactobacillus mixture comprising Pediococcus acidilactici, Pediococcus pentosaceus , and Lactobacillus plantarum.

12. The composition of claim 11 , wherein the remainder of the composition comprise an oxidizable carbon source.

13. The composition of claim 12 , wherein the oxidizable carbon source is anhydrous dextrose or dextrose monohydrate.

14. The composition of claim 11 , wherein the Lactobacillus in the Lactobacillus mixture are present in equal proportions by weight.

15. The composition of claim 11 , the wherein the Bacillus subtilis subsp mojavensis , the Bacillus amyloliquefaciens , and the Bacillus pumilus in the first Bacillus mixture are present in equal proportions by weight.

Assignments (3)
RELEASE OF SECURITY INTEREST Recorded Aug 8, 2022
From: BARGA, LLC, AS COLLATERAL AGENT
To: BIOWISH TECHNOLOGIES, INC., ASSIGNOR
Reel/Frame 060748/0136 →
SECURITY INTEREST Recorded May 15, 2020
From: BIOWISH TECHNOLOGIES INTERNATIONAL, INC. (A DELAWARE CORPORATION)
To: BARGA, LLC (A TEXAS LIMITED LIABILITY COMPANY)
Reel/Frame 052679/0764 →
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Feb 11, 2016
From: SHOWELL, MICHAEL S.; CARPENTER, RICHARD S.; GORSUCH, JOHN; ROBERTS, JOSEPH; BARTOLI, DAVID; ROSENE, RICHARD; ROSENE, LORA
To: BIOWISH TECHNOLOGIES, INC.
Reel/Frame 037714/0462 →
Continuity (2)
Provisional Application 62073335 · Oct 31, 2014
Provisional Application 62101741 · Jan 9, 2015