Method of diagnosing and treating melioidosis
View Patent ↗Non-invasive methods are provided herein for the diagnosis of melioidosis with specific antibodies capable of detecting molecules associated with melioidosis in a biological fluid, such as urine or serum. These molecules can be identified using proteomic methods, including but not limited to antibody based methods, such as an enzyme-linked immunosorbant assay (ELISA), a radioimmunoassay (RIA), or a lateral flow immunoassay. Methods of inducing an immune response to melioidosis are also disclosed. The methods include the use of the immunogenic melioidosis polypeptides, nucleic acids encoding these polypeptides, and/or viral vectors encoding an immunogenic melioidosis polypeptide, alone or in conjunction with other agents, such as traditional melioidosis therapies. Also disclosed are methods for treating a subject having melioidosis. These methods include inducing an immune response to melioidosis and/or using an inhibitory nucleic acid, such as a siRNA or antisense molecule, to decrease a melioidosis associated molecule expression in order to treat melioidosis.
1. A method of diagnosing a subject with melioidosis, comprising:
detecting, with an antibody produced by immunizing an animal with a melioidosis-associated antigen, by laboratory immunoassay, the melioidosis-associated antigen in a sample obtained from a subject exhibiting one or more signs or symptoms associated with melioidosis or a subject known to be at risk of acquiring melioidosis so as to obtain laboratory assay results regarding the presence of the melioidosis-associated antigen in the sample, wherein the melioidosis-associated antigen is a capsular polysaccharide (CPS) comprising an unbranched homopolymer of 6-deoxy-D-manno-heptose; and
providing laboratory assay results thereby diagnosing whether the subject has melioidosis.
2. The method of claim 1 , further comprising comparing detection of the melioidosis-associated antigen in the sample obtained from the subject exhibiting one or more signs or symptoms associated with melioidosis to a control, wherein increased detection of the melioidosis-associated antigen relative to a control indicates that the subject has melioidosis.
3. The method of claim 2 , wherein the method is used for diagnosing a subject with acute melioidosis.
4. The method of claim 2 , wherein detecting at least one melioidosis-associated antigen comprises using a lateral flow device.
5. The method of claim 1 , wherein the method is used for detecting any condition or disease associated with B. mallei, B. pseudomallei, B. thailandensis or a combination thereof.
6. The method of claim 1 , wherein the sample is a urine sample.
7. A method of monitoring the efficacy of a melioidosis therapy, comprising:
detecting, with an antibody produced by immunizing an animal with a melioidosis-associated antigen, by laboratory immunoassay, the melioidosis-associated antigen in a sample obtained from a subject that has been administered a meliodosis therapy, wherein the melioidosis-associated antigen is a capsular polysaccharide (CPS) comprising an unbranched homopolymer of 6-deoxy-D-manno-heptose;
comparing detection of the melioidosis-associated antigen in the sample obtained from the subject to a reference sample;
providing laboratory assay results thereby monitoring the efficacy of the melioidosis therapy.
8. The method of claim 7 , wherein the method is used for monitoring any condition or disease associated with B. mallei, B. pseudomallei, B. thailandensis or a combination thereof.
9. The method of claim 7 , wherein the reference sample is a sample from a subject with melioidosis.
10. The method of claim 7 , wherein the reference sample is a sample from a subject that does not have melioidosis.
11. The method of claim 1 , wherein the antibody is produced by a hybridoma created from the immunized animal.
12. The method of claim 7 , wherein the antibody is produced by a hybridoma created from the immunized animal.
13. The method of claim 1 , wherein detecting comprises detecting a plurality of melioidosis-associated antigens.
14. The method of claim 7 , wherein detecting comprises detecting a plurality of melioidosis-associated antigens.
15. The method of claim 1 , wherein the antibody is a monoclonal antibody.
16. The method of claim 7 , wherein the antibody is a monoclonal antibody.