Liposome targeting compounds and related uses
The present invention provides liposome targeting compounds for targeting cells expressing sialic acid binding Ig-like lectin (Siglec). The liposome compounds typically comprise a binding moiety that is a glycan ligand of the Siglec on the target cell. The liposome compounds also incorporate or encapsulate a biological agent (e.g., an antigen or a therapeutic agent) that can be delivered to the target cell to modulate or kill the target cell. The invention also provides methods of using the liposome targeting compounds for targeted delivery of antigen and for modulating immune cells or immune responses.
1. A method for activating an immune response in T cells to a polypeptide or glycolipid antigen, comprising
(a) providing a liposome targeting compound comprising a liposome and a glycan ligand for a Siglec on a human or mouse macrophage or dendritic cell, wherein the glycan ligand is displayed on the liposome and the polypeptide or glycolipid antigen is incorporated into the liposome; and
(b) contacting the liposome targeting compound with the macrophage or dendritic cell;
thereby activating an immune response in T cells to the antigen.
2. The method of claim 1 , wherein the activated immune response in T cells is activation of CD4 + and CD8 + T cells.
3. The method of claim 1 , wherein the glycolipid antigen is an activator of natural killer T (NKT) cells, and wherein the activated immune response in T cells is activation of NKT cells.
4. The method of claim 1 , wherein the liposome targeting compound is contacted with the macrophage or dendritic cell in vitro.
5. The method of claim 1 , wherein the liposome targeting compound is administered to a subject and contacted with the macrophage or dendritic cell inside the body of the subject.
6. The method of claim 1 , wherein the Siglec is sialoadhesin.
7. The method of claim 1 , wherein the glycan ligand is 9-N-biphenylcarboxyl-NeuAcα2-3Galβ1-4GlcNAc (3′- BPC NeuAc).