Feedback-resistant alpha-isopropylmalate synthases
The invention relates to an isolated nucleotide sequence encoding an amino acid sequence that is at least ≧90%, ≧92%, ≧94%, ≧96%, ≧97%, ≧98%, ≧99% or 100%, preferably ≧97%, particularly preferably ≧98%, very particularly preferably ≧99%, and extremely preferably 0%, identical to the amino acid sequence of SEQ ID NO:2, wherein SEQ ID NO:2, at position 553, or at a corresponding position of the amino acid sequence, has a proteinogenic amino acid other than L-tyrosine, to a microorganism comprising the nucleotide sequence and also to a process for producing fine chemicals using this microorganism.
1. An isolated polynucleotide encoding an α-isopropylmalate synthase comprising an amino acid sequence that is at least 90% identical to the amino acid sequence of SEQ ID NO:2, wherein the amino acid sequence has a proteinogenic amino acid other than L-tyrosine at position 553 of SEQ ID NO:2 or at a corresponding position of the amino acid sequence.
2. An isolated polynucleotide encoding an α-isopropylmalate synthase comprising an amino acid sequence that is at least 90%, identical to the amino acid sequence of SEQ ID NO:2, wherein the amino acid sequence has a proteinogenic amino acid other than L-tyrosine at position 553 of SEQ ID NO:2 or at a corresponding position of the amino acid sequence and the proteinogenic amino acid is selected from the group consisting of glutamic acid, aspartic acid, alanine, cysteine, serine, threonine, lysine, arginine, glutamine and asparagine.
3. The isolated polynucleotide according to claim 1 , wherein the amino acid sequence encoded thereby has, at position 553 of SEQ ID NO:2 or at a corresponding position of the amino acid sequence, L-aspartic acid.
4. The isolated polynucleotide according to claim 1 , having guanine at position 1657 of SEQ ID NO:5 or at a corresponding position of the polynucleotide.
5. The isolated polynucleotide according to claim 1 , as depicted in SEQ ID NO: 5.
6. Vector comprising the isolated polynucleotide according to claim 1 .
7. Vector comprising the isolated polynucleotide according to claim 2 , which is suitable for replication in microorganisms of the genus Corynebacterium.
8. Polypeptide comprising an amino acid sequence encoded by the isolated polynucleotide according to claim 1 .
9. Microorganism of the genus Corynebacterium comprising the isolated polynucleotide according to claim 1 or a polypeptide comprising an amino acid sequence encoded by said isolated polynucleotide or a vector comprising said isolated polynucleotide.
10. Microorganism according to claim 9 , in which said isolated polynucleotide is present in overexpressed form as compared with a starting strain or wild-type strain.
11. Microorganism according to claim 9 , wherein the isolated polynucleotide is integrated in a chromosome.
12. Microorganism according to claim 9 , wherein it is Corynebacterium glutamicum.
13. Microorganism according to claim 9 , wherein the microorganism has the capability of producing L-leucine or ketoisocaproate (KIC).
14. Fermentative process for producing KIC or L-leucine comprising the following steps:
a) fermenting of one of the microorganisms according to claim 9 in a medium,
b) accumulating the KIC or L-leucine in the medium, wherein a fermentation broth is obtained.
15. Process according to claim 14 , wherein it is a process which is selected from the group consisting of batch process, fed-batch process, repetitive fed-batch process and continuous process.
16. Process according to claim 14 , wherein the KIC or L-leucine is obtained from the fermentation broth.
17. A method for the fermentative production of L-leucine or KIC comprising culturing the microorganism of claim 9 .
18. Microorganism of the genus Corynebacterium comprising the isolated polynucleotide according to claim 2 or a polypeptide comprising an amino acid sequence encoded by said isolated polynucleotide or a vector comprising said isolated polynucleotide.
19. Microorganism according to claim 18 , in which said isolated polynucleotide is present in overexpressed form as compared with a starting strain or wild-type strain.