Compositions, apparatus and methods for monitoring biomarkers

1. A method for detecting the presence of nitric oxide in saliva wherein the device comprises a single unit for collecting the saliva, analyzing the contents of the saliva, and correlating the analysis with blood pressure,

wherein the single unit comprises an elongated strip, wherein the strip contains a scored mark at the midpoint of the strip and wherein the strip contains a first absorbent pad at one end and a second absorbent pad at the opposite end,

wherein the first absorbent pad comprises a fluid collection pad, and wherein the second absorbent pad comprises a test pad,

and wherein the test pad enables dry reagent detection chemistry comprising components modified from the Griess diazotization reaction, comprising mixture of N-naphthylenediamine-dihydrochloride and sulphanilamide, or N-(naphthyl)ethylenediammonium-dihydrochloride and sulphanilamide, or para-arsanilic acid N-ethylenediamine tetrahydroquinoline, or para-arsanilic acid N-(1-naphthyl)ethylenediamine dihydrochoride;

wherein the biomarker comprises nitric oxide analyte, nitrite, and wherein the fluid collection pad comprises a wicking pad,

wherein the fluid collection pad is inserted into the mouth for a first predetermined period of time to absorb saliva, wherein the device is then folded so that the fluid collection pad and test pad make contact for a second predetermined period of time, wherein the contact between the fluid collection pad and test pad results in a color change on the test pad following a third predetermined period,

wherein the color change is compared to a color scale and wherever the color falls within the scale, a representative physiological nitric oxide content is evaluated; and

wherein the test pad provides a concentration range of the nitric oxide analyte, nitrite, ranging from 12 to >400 umol/L with visibly distinct color ranges corresponding to 0 to 25, 25 to 100, 100 to 200, 200 to 350, or greater than 400 umol/L; wherein saliva nitrite levels greater than 400 umol/L correlate with a reduction in blood pressure.

2. The method of claim 1 , wherein the fluid collection pad comprises a wicking pad, membrane, paper, resin, sponge, immunoabsorbent pad, ionic, or other suitable platform that absorbs saliva analytes to be transferred to the test reagent pad.

3. The method of claim 1 , wherein the representative physiological nitric oxide content is utilized to enable the assessment of vascular wellness and fitness.

4. The method of claim 3 , wherein vascular wellness and fitness comprises the ability to maintain a normal blood pressure range, lower blood pressure, extend time-to-exhaustion, reduce the need for oxygen consumption, improve cellular respiration, or mitochondrial efficiency.

5. The method of claim 1 , wherein the representative physiological nitric oxide content is utilized to enable modification of nitric oxide levels comprising daily physical exercise, anaerobic training, aerobic training, consuming a diet consisting of leafy green vegetables and other nitrate-rich vegetables, fruit, whole foods supplement intended to increase nitric oxide levels, consuming a Mediterranean diet, or consuming a DASH diet.

6. The method of claim 1 , wherein the first and second predetermined period of time are about 10 seconds; and wherein the third predetermined period of time is about 10-45 seconds.