Identification of secreted proteins as detection markers for citrus disease
View Patent ↗Secreted proteins as detection markers for insect vector and graft transmitted citrus disease are described. Method and kits for detecting the secreted proteins are provided.
1. A method of detecting the presence of CLIBASIA_05315 protein (SEQ ID NO:29) in a citrus plant, the method comprising:
obtaining sap from the citrus plant;
contacting the sap with an antibody that specifically binds the protein consisting of SEQ ID NO:29;
detecting the presence or absence of specific binding of the antibody to SEQ ID NO:29 in the sap, thereby detecting the presence or absence of SEQ ID NO:29 in the citrus plant; and
destroying the citrus plant when SEQ ID NO:29 is detected in the citrus plant.
2. The method of claim 1 , wherein the citrus plant is not artificially infected or grafting-inoculated with a bacterial pathogen.
3. The method of claim 1 , wherein samples of sap from a plurality of citrus plants are screened by the method.
4. The method of claim 1 , wherein the sap is applied to a membrane and the detecting of binding comprises contacting the antibody to the membrane.
5. The method of claim 1 , wherein the antibody is linked to a solid support.
6. The method of claim 1 , wherein the sap is unpurified.