Thermal cycling apparatus and method
A system for holding at least one of sample and reagent for analysis. The system includes a pair of parallel covers, at least one of which is light transmissive, of which pair a light transmissive cover forms a top, and of which pair the other forms a bottom. A frame is disposed between the covers to define, in relation to the covers, an interior volume. The frame and the covers are associated with one another to form a case, the case being substantially tight to liquids. A microfluidic array is disposed in the interior volume. The array includes a sheet of material having a pair of opposed surfaces, a thickness, and a plurality of through-holes running through the thickness between the surfaces, the through-holes containing at least one of sample and reagent.
1. A system for analyzing a PCR reaction, comprising:
a sample holder comprising a plurality of reaction volumes configured to receive a corresponding plurality of samples, each reaction volume having a volume of less than 100 nanoliters, the reaction volumes being spatially separated from one another;
a case comprising a case volume and an opening, the case configured to contain the sample holder within the case volume, the opening configured for dispensing a liquid into the case volume;
an imaging system comprising an illumination source, the imaging system configured to image the plurality of samples; and
a processor comprising analysis software configured to perform an end-point PCR analysis on at least some of the plurality of samples;
wherein the sample holder comprises a sheet of material comprising a pair of opposing surfaces, the plurality of reaction volumes comprises a plurality of through-holes, and the plurality of through-holes are disposed between the opposing surfaces.
2. The system of claim 1 , wherein the illumination source is configured to simultaneously illuminate an entirety of the reaction volumes.
3. The system of claim 1 , wherein the illumination source comprises a light emitting diode.
4. The system of claim 1 , wherein the end-point PCR analysis comprises genotyping.
5. The system of claim 1 , wherein the reaction volumes have a density greater than one reaction volume per 0.25 mm 2 .
6. The system of claim 1 , wherein the sample holder comprises at least 3072 reaction volumes, the plurality of samples being disposed within respective ones of the 3072 reaction volumes.
7. The system of claim 1 , further comprising a thermal cycler configured to cycle the plurality of samples over a temperature range.
8. The system of claim 1 , wherein the system further comprises a liquid, wherein the liquid comprises at least one of a mineral oil, a silicon oil, or a perfluorinated hydrocarbon.
9. The system of claim 1 , wherein the analysis software is configured to perform a quantitative PCR.