Loading system for an encapsulation device
The present invention provides, in at least one embodiment, a loading device, system and method that loads aggregate cells into an encapsulation device for implanting into a patient. The loading system uses negative pressure from a low pressure pump in a closed system to improve safety and cell viability while allowing for even loading of the encapsulation device.
1. A cell encapsulation loading system comprising:
a) a cell encapsulation loading device comprising
(1) a housing comprising a first housing member and a second housing member, wherein the first housing member is coupled to the second housing member, thereby forming a hollow device chamber and
(2) an encapsulation device comprising an inlet port wherein the inlet port is detachably coupled to the housing and the encapsulation device is enclosed within the hollow device chamber; and
b) an inverting holding rack for tilting or inverting the cell encapsulation loading device during loading.
2. The cell encapsulation loading system of claim 1 , wherein the housing further comprises a first port.
3. The cell encapsulation loading system of claim 2 , wherein the housing further comprises a second port.
4. The cell encapsulation loading system of claim 3 , wherein the second port is adapted for receiving negative pressure.
5. The cell encapsulation loading device system of claim 2 , wherein the first port is adapted for sterilely loading cells into the encapsulation device.
6. The cell encapsulation loading system of claim 1 , wherein the encapsulation device further comprises living cells.
7. The cell encapsulation loading system of claim 1 , wherein the first housing member and the second housing member are detachably coupled.
8. The cell encapsulation loading system of claim 1 , further comprising:
a cell chamber containing living cells and fluid medium, wherein the cell chamber is in fluid communication with the encapsulation loading device.
9. The cell encapsulation loading system of claim 8 , wherein the living cells and fluid media are loaded into the encapsulation device through the inlet port.
10. The cell encapsulation loading system of claim 8 , further comprising at least one fluid reservoir that is in fluid communication with the loading device for dispensing sterile fluid to the encapsulation device, wherein the reservoir is selected from the group consisting of a bottle, a flask, a funnel, a tube and a bag.
11. The cell encapsulation loading system of claim 8 , further comprising a computer for automating a process of loading the encapsulation device with the living cells and fluid medium.
12. The cell encapsulation loading system of claim 1 , wherein the inverting holding rack is coupled to a motor for automating inversion of the cell loading device.
13. The cell encapsulation loading system of claim 8 , further comprising a means for maintaining the cells in suspension during loading.
14. The cell encapsulation loading system of claim 13 , wherein the means for maintaining the cells in suspension during loading is an orbital rotator.
15. The cell encapsulation loading system of claim 1 , further comprising a means for loading cells using negative pressure.
16. A method of loading an encapsulation device, the method comprising the steps of:
a) providing a cell encapsulation loading system according to claim 1 ;
b) providing a cell chamber comprising living cells and a fluid medium; and
c) applying negative pressure to draw the living cells and the fluid medium through the inlet port and into the encapsulation device, thereby loading the encapsulation device.
17. The method of claim 16 , wherein the method is semi-automated.