Surgical sutures incorporated with stem cells or other bioactive materials
Materials and methods for immobilizing bioactive molecules, stem and other precursor cells, and other agents of therapeutic value in surgical sutures and other tissue scaffold devices are described herein. Broadly drawn to the integration and incorporation of bioactive materials into suture constructs, tissue scaffolds and medical devices, the present invention has particular utility in the development of novel systems that enable medical personnel performing surgical and other medical procedures to utilize and subsequently reintroduce bioactive materials extracted from a patient (or their allogenic equivalents) to a wound or target surgical site.
1. A method of fabricating a surgical suture, the method comprising:
providing a suture element in an open state, the suture element comprising a plurality of filaments configured in at least one of a braided and a woven configuration to define a plurality of pores or interstices, the plurality of pores or interstices having at least one first size and shape in the open state sufficient to permit a biological cell to traverse the pores or interstices;
causing a plurality of the biological cells to traverse the plurality of pores or interstices so as to move from an exterior of the suture element to an interior core of the suture element; and
manipulating the suture element to a closed state, the manipulation of the suture element deforming the plurality of pores or interstices to at least one second size and shape in the closed state, the at least one second size and shape sufficient to restrict migration of the plurality of biological cells from the interior core of the suture element to the exterior of the suture element through the plurality of pores or interstices.
2. The method of claim 1 , wherein manipulating the suture element from the open state to the closed state causes a change in a diameter of the suture element.
3. The method of claim 2 , wherein the diameter of the suture element decreases.
4. The method of claim 3 , wherein decreasing the diameter of the suture element increases a length of the suture element.
5. The method of claim 1 , wherein plurality of filaments comprise a rapidly absorbable filament and a structural filament of reduced absorbability.
6. A method of loading biological cells in a surgical suture comprising:
providing a surgical suture of porous, braided or woven structure characterized by pores or interstices in an exterior surface of the surgical suture, which permit flow of biological cells into an interior of the surgical suture in a first state;
inducing a biological cell containing media to migrate into the interior of said suture; and
inducing the pores or interstices in the exterior surface of the surgical suture to a second state where migration of biological cells is inhibited.
7. The method of claim 6 , wherein the step of inducing said pores or interstices to a second state includes addition of a biodegradable material into the pores or interstices of the external surface of said suture.
8. The method of claim 6 , wherein the step of inducing said pores or interstices to a second state includes deforming the size or shape of said pores or interstices such that at least one dimension of said pores or interstices is reduced and the migration of biological cells is inhibited.
9. The method of claim 6 , wherein the wherein the step of inducing said pores or interstices to a second state includes increasing the length of a section of the suture.
10. A method of loading biological cells in a surgical suture comprising:
providing a surgical suture of porous, braided or woven structure characterized by pores or interstices which permit flow of biological cells into the interior core of said suture;
inducing a biological cell containing media to migrate into the pores or interstices of the interior core; and
manipulating the surgical suture by applying force along or around a longitudinal axis of the surgical suture such that the diameter of the surgical suture decreases.