Synthesis of long-chain polyunsaturated fatty acids by recombinant cell
The present invention relates to methods of synthesizing long-chain polyunsaturated fatty acids, especially eicosapentaenoic acid, docosapentaenoic acid and docosahexaenoic acid, in recombinant cells such as yeast or plant cells. Also provided are recombinant cells or plants which produce long-chain polyunsaturated fatty acids. Furthermore, the present invention relates to a group of new enzymes which possess desatorase or elongase activity that can be used in methods of synthesizing long-chain polyunsaturated fatty acids.
1. A cell comprising eicosapentaenoic acid in an esterified form as part of triacylglycerols in the cell and an exogenous polypeptide having Δ5 desaturase activity whose amino acid sequence is at least 90% identical to the amino acid sequence set forth as SEQ ID NO: 60.
2. The cell of claim 1 , wherein at least 50% of the eicosapentaenoic acid in the cell is incorporated into triacylglycerols.
3. The cell of claim 1 , further comprising docosahexaenoic acid in an esterified form as part of triacylglycerols in the cell, wherein at least 50% of the docosahexaenoic acid in the cell is incorporated into triacylglycerols.
4. The cell of claim 1 , wherein the total fatty acid content in the cell comprises 2.5% (w/w) C20 ω3 long-chain polyunsaturated fatty acids (LC-PUFAs) and 0.1% (w/w) docosapentaenoic acid.
5. The cell of claim 4 , wherein the total fatty acid content in the cell comprises 0.13% (w/w) docosapentaenoic acid.
6. The cell of claim 5 , wherein the total fatty acid content in the cell comprises 0.5% (w/w) docosapentaenoic acid.
7. The cell of claim 1 , wherein the amino acid sequence of the exogenous polypeptide is identical to the amino acid sequence set forth as SEQ ID NO: 60.
8. A process of producing oil comprising
i) obtaining the cell of claim 1 , and
ii) extracting oil from the cell.
9. The process of claim 8 , wherein the cell is a plant cell.
10. The cell of claim 1 , wherein the amino acid sequence of the exogenous polypeptide is at least 95% identical to the amino acid sequence set forth as SEQ ID NO: 60.
11. The cell of claim 1 , wherein the amino acid sequence of the exogenous polypeptide is at least 99% identical to the amino acid sequence set forth as SEQ ID NO: 60.