Compositions and methods for treating cancer
A composition for inhibiting cancer cell proliferation includes exogenous constitutively active Chk1 or an agent that promotes phosphorylation of endogenous Chk1 of the cancer cell.
1. A composition for inhibiting cancer cell proliferation, said composition comprising a vector including a nucleic acid encoding a polypeptide that promotes phosphorylation of endogenous Chk1 of a cancer cell, wherein the polypeptide inhibits cancer cell proliferation in the absence of exogenous DNA damage, wherein when the vector expresses the nucleic acid encoding the polypeptide, the expressed polypeptide consists of a C-terminal fragment of Chk1 having an amino acid sequence with at least 80% sequence identity to amino acids 331-476 of Chk1 or amino acids 421-476 of Chk1 of SEQ ID NO: 2.
2. The composition of claim 1 , wherein the composition further comprises a pharmaceutically effective carrier.
3. The composition of claim 1 , wherein the polypeptide consists of SEQ ID NO: 4 or SEQ ID NO: 5.
4. A method for inhibiting cancer cell proliferation, comprising: expressing in the cancer cell a therapeutically effective amount of a polypeptide that promotes phosphorylation of endogenous Chk1 of the cancer cell, wherein the expressed polypeptide consists of a C-terminal fragment of Chk1 having an amino acid sequence with at least 90% sequence identity to amino acids 331-476 of Chk1 or amino acids 421-476 of Chk1 of SEQ ID NO: 2.
5. The method of claim 4 , wherein the polypeptide consists of SEQ ID NO: 4 or SEQ ID NO: 5.