Resistance gene and uses thereof
The invention provides methods for producing a plant with altered resistance to powdery mildew, the methods comprising transformation of a plant with a genetic construct including a polynucleotide encoding of a polypeptide with the amino acid sequence of SEQ ID NO: 1 or a variant of fragment thereof. The invention also provides isolated polypeptides, polynucleotides, constructs and vectors useful for producing a plant cell and plants transformed to contain and express the polypeptides, polynucleotides and constructs. The invention also provides plants produced by methods of the invention.
1. A host cell, plant cell, plant, plant part or plant propagule, that contains a transgene comprising a polynucleotide encoding a polypeptide, said polypeptide comprising a sequence with at least 98% identity to the amino acid sequence of at least one of:
a) SEQ ID NO: 1,
b) SEQ ID NO: 5, and
c) SEQ ID NO: 6;
wherein the polypeptide confers resistance to the powdery mildew fungus Podosphaera leucotricha in a Rosaceae plant.
2. The host cell, plant cell, plant, plant part, or plant propagule, of claim 1 , wherein the polypeptide comprises the amino acid sequence of at least one of:
a) SEQ ID NO: 1,
b) SEQ ID NO: 5, and
c) SEQ ID NO: 6.
3. The host cell, plant cell, plant, plant part, or plant propagule, of claim 1 wherein the polypeptide comprises the amino acid sequence of SEQ ID NO: 1.
4. The host cell, plant cell, plant, plant part, or plant propagule, of claim 1 wherein the polypeptide comprises the amino acid sequence of SEQ ID NO: 5.
5. The host cell, plant cell, plant, plant part, or plant propagule, of claim 1 wherein the polypeptide comprises the amino acid sequence of SEQ ID NO: 6.
6. The host cell, plant cell, plant, plant part, or plant propagule, of claim 1 , wherein the polynucleotide comprises the sequence with at least 95% identity to at least one of:
a) SEQ ID NO: 2,
b) SEQ ID NO: 3,
c) SEQ ID NO: 7, and
d) SEQ ID NO: 8.
7. The host cell, plant cell, plant, plant part, or plant propagule, of claim 1 , wherein the polynucleotide comprises the sequence of at least one of:
a) SEQ ID NO: 2,
b) SEQ ID NO: 3,
c) SEQ ID NO: 7, and
d) SEQ ID NO: 8.
8. An expression construct comprising a promoter operably linked to a polynucleotide encoding a polypeptide, said polypeptide comprising a sequence with at least 98% identity to the amino acid sequence of at least one of:
a) SEQ ID NO: 1
b) SEQ ID NO: 5, and
c) SEQ ID NO: 6;
wherein the polypeptide confers resistance to the powdery mildew fungus Podosphaera leucotricha in a Rosaceae plant, and wherein the promoter is heterologous to the polynucleotide.
9. A host cell, plant cell, plant, plant part, or plant propagule, comprising the expression construct of claim 8 .
10. A method for producing a plant cell or plant with increased resistance to powdery mildew, the method comprising transforming a plant cell or plant with a polynucleotide encoding a polypeptide, said polypeptide comprising a sequence with at least 98% identity to the amino acid sequence of at least one of:
a) SEQ ID NO: 1,
b) SEQ ID NO: 5, and
c) SEQ ID NO: 6;
wherein the polypeptide confers resistance to the powdery mildew fungus Podosphaera leucotricha in a Rosaceae plant.
11. The method of claim 10 , wherein the polynucleotide is part of an expression construct.
12. A method for producing a progeny of the plant of claim 1 with increased resistance to powdery mildew, the method comprising at least one of:
a) breeding using the plant as a male or female parent to produce the progeny plant, and
b) asexually reproducing the plant to produce the progeny plant;
wherein the progeny plant contains the transgene.
13. A method for producing a plant cell or plant with increased resistance to powdery mildew, the method comprising transformation of a plant cell or plant comprising a polynucleotide encoding a polypeptide, said polypeptide comprising a sequence with at least 95% identity to the amino acid sequence of at least one of:
a) SEQ ID NO: 1,
b) SEQ ID NO: 5, and
c) SEQ ID NO: 6;
wherein the polypeptide confers resistance to the powdery mildew fungus Podosphaera leucotricha in a Rosaceae plant.
14. A method for producing a polypeptide conferring resistance to powdery mildew, the method comprising expressing in a plant cell or plant a polynucleotide encoding a polypeptide, said polypeptide comprising a sequence with at least 95% identity to the amino acid sequence of at least one of:
a) SEQ ID NO: 1,
b) SEQ ID NO: 5, and
c) SEQ ID NO: 6;
wherein the polypeptide confers resistance to the powdery mildew fungus Podosphaera leucotricha in a Rosaceae plant.