Method for diagnosing cancer by detecting GPC3
Provided is a method for diagnosing cancer by detecting a novel cancer marker. Cancer can be diagnosed by detecting soluble glypican 3 in a test sample.
1. A sandwich ELISA kit for diagnosing a possible presence of GPC3 protein expressing hepatic cancer in a human subject by detecting a soluble GPC3 protein level in a test sample obtained from the human subject, comprising two types of anti-GPC3 antibodies each recognizing the N-terminal peptide of GPC3, wherein the N-terminal peptide of GPC3 is a peptide fragment consisting of the amino acid sequence from the 1st amino acid to the 358th amino acid of SEQ ID NO: 4.
2. The kit of claim 1 , wherein the test sample is selected from the group consisting of blood, serum and plasma.
3. The kit of claim 2 , wherein the detected soluble GPC3 level in the test sample is greater than a control level of GPC3 in normal non-cancerous blood, serum or plasma, indicates the possible presence of GPC3 protein expressing hepatic cancer in the human subject.
4. The kit of claim 1 , comprising an anti-GPC3 antibody immobilized on a carrier and an anti-GPC3 antibody labeled with a labeling substances.
5. The kit of claim 4 , wherein the labeling substances are biotin.