Manipulation of flavonoid biosynthesis in plants
The present invention relates to nucleic acids and nucleic acid fragments encoding amino acid sequences for flavonoid biosynthetic enzymes in plants, and the use thereof for the modification of flavonoid biosynthesis in plants. More particularly, the flavonoid biosynthetic enzyme is selected from the group consisting of chalcone isomerase (CHI), chalcone synthase (CHS), chalcone reductase (CHR), dihydroflavonol 4-reductase (DFR), leucoanthocyanidin reductase (LCR), flavonoid 3′,5′ hydrolase (F3′5′H), flavanone 3-hydroxylase (F3H), flavonoid 3′-hydroxylase (F3′H), phenylalanine ammonia-olyase (PAL) and vestitone reductase (VR), and functionally active fragments and variants thereof.
1. A substantially purified or isolated nucleic acid encoding chalcone synthase, wherein said nucleic acid comprises the entirety of a nucleotide sequence selected from the group consisting of SEQ ID NOs: 23 and 313.
2. A construct comprising the nucleic acid according to claim 1 .
3. A vector comprising the nucleic acid according to claim 1 .
4. The vector according to claim 3 , further comprising a promoter and a terminator, said promoter, nucleic acid and terminator being operatively linked.
5. A plant cell, plant, plant seed or other plant part, transformed with the vector according to claim 3 .
6. A method selected from the group consisting of:
(a) modifying flavonoid biosynthesis in a plant;
(b) modifying UV-light absorption in a plant;
(c) modifying pigment production in a plant; and
(d) modifying plant defense to a biotic stress;
said method comprising introducing into said plant the nucleic acid according to claim 1 .
7. A plant cell, plant, plant seed or other plant part, transformed with the construct according to claim 2 .