Bacteria-targeted magnetic resonance contrast agents
The present invention relates to bacteria-targeted contrast agents for magnetic resonance imaging (MRI). In particular, the present invention relates to bacteria targeted MRI contrast agents that can be used to detect bacteria in vivo or in vitro. In certain embodiments, the contrast agents comprise a metal chelate conjugated to at least two Zn-dipicolylamine groups.
1. A method for imaging bacteria in a subject comprising:
a) administering a contrast agent to a subject, wherein said contrast agent is selected from the group consisting of:
wherein M is a metal ion, and wherein R is a linker moiety selected from the group consisting of:
i) an amino acid,
ii) a peptide,
iii) a divalent branched or unbranched carbon chain comprising from about 2 to about 30 carbon atoms,
iv) a divalent branched or unbranched carbon chain comprising from about 2 to about 30 carbon atoms, which the chain is substituted with one or more hydroxyl groups or oxo groups,
v) a divalent branched or unbranched carbon chain comprising from about 2 to about 30 carbon atoms, wherein one or more of the carbon atoms in the chain is replaced with an aryl ring, a hetoroaryl ring, or with a non-peroxide —O—, —S— or —NH—,
vi) —C(═O)NH(CH 2 ) 3 —;
vii) —C(═O)NH(CH 2 ) 5 C(═O)NH(CH 2 )—;
viii —CH 2 OC(═O)NH(CH 2 ) 2 O(CH 2 ) 2 O(CH 2 )—, and
ix) a divalent heteroaryl group, and
b) performing imaging of said subject and detecting a signal from said contrast agent if said subject is infected with bacteria.
2. The method of claim 1 , wherein said bacteria comprises E. coli.
3. The method of claim 1 , wherein said bacteria comprises Staphylococcus bacteria.
4. The method of claim 1 , wherein said imaging comprises magnetic resonance imaging.