Inhalable liquid formulations of DNase I
An inhalable pharmaceutical composition for pulmonary administration comprising human DNase I and a liquid carrier and, more particularly, but not exclusively, to methods, liquid pharmaceutical inhalation devices and systems for the therapeutic use thereof are provided.
1. An inhalable pharmaceutical composition for pulmonary administration comprising a human DNase I protein and a physiologically acceptable pharmacologically-inert liquid carrier, wherein said human DNase I protein is a plant expressed recombinant human DNase I protein comprising an N-terminal Glycine residue at position 1 of said human DNase I protein.
2. The inhalable pharmaceutical composition of claim 1 , wherein said human DNase I protein comprising an N-terminal Glycine residue at position 1 of said human DNase I protein comprises the amino acid sequence as set forth in SEQ ID NO:6.
3. The inhalable pharmaceutical composition of claim 1 , wherein said human DNase I protein comprises the amino acid sequence as set forth in SEQ ID NO:5.
4. The inhalable pharmaceutical composition of claim 1 , wherein said human DNase I protein has at least one core α-(1,3) fucose.
5. The inhalable pharmaceutical composition of claim 1 , wherein said human DNase I protein has reduced susceptibility to actin inhibition of endonuclease activity as compared with that of mammalian cell expressed human recombinant DNase I.
6. The inhalable pharmaceutical composition of claim 1 , further comprising, as an active ingredient, a magnesium salt.
7. The inhalable pharmaceutical composition of claim 1 , further comprising, as an active ingredient, an agent for inhibiting formation of G actin and/or enhancing formation of F actin.
8. The inhalable pharmaceutical composition of claim 1 , wherein said human DNase I protein is formulated in liposomes.
9. The inhalable pharmaceutical composition of claim 1 , wherein said human DNase I protein is at least 90-95% pure human DNase I protein.
10. The inhalable pharmaceutical composition of claim 1 , further comprising plant beta-acetylhexosaminidase enzyme protein.
11. The inhalable pharmaceutical composition of claim 9 , wherein said plant beta-acetylhexosaminidase enzyme protein is inactivated beta-acetylhexosaminidase enzyme protein.
12. A method of reducing extraceullular DNA in a secretion, fluid or tissue of a subject in need thereof, the method comprising administering to the subject the inhalable pharmaceutical composition of claim 1 , thereby reducing extraceullular DNA in the secretion, fluid or tissue of the subject.
13. The method of claim 12 , wherein said subject is suffering from a disease or condition selected from the group consisting of male infertility, metastatic cancer, a viral, bacterial, fungal or protozoan infection, sepsis, atherosclerosis, diabetes, delayed type hypersensitivity and a uterine disorder.
14. A method of reducing DNA in a pulmonary secretion or fluid of a subject in need thereof, the method comprising administering to the subject the inhalable pharmaceutical composition of claim 1 , thereby reducing DNA in the pulmonary secretion or fluid of the subject.
15. A method of treating a pulmonary disease or condition associated with excess DNA in a pulmonary secretion in a subject in need thereof, the method comprising administering to the subject the inhalable pharmaceutical composition of claim 1 , thereby treating the pulmonary disease or condition associated with excess DNA in the pulmonary secretion in the subject.
16. The method of claim 14 , wherein said subject is suffering from a respiratory disease selected from the group consisting of acute or chronic bronchopulmonary disease, atelectasis due to tracheal or bronchial impaction, and complications of tracheostomy.
17. The method of claim 16 , wherein said acute or chronic bronchopulmonary disease is selected from the group consisting of infectious pneumonia, bronchitis or tracheobronchitis, bronchiectasis, cystic fibrosis, asthma, chronic obstructive pulmonary disease (COPD), TB or fungal infections.
18. The method of claim 15 , wherein an effective amount of said inhalable pharmaceutical composition is a single dose of 0.1 to 25 mg DNase I, administered daily.
19. The method of claim 17 , wherein an effective amount of said inhalable pharmaceutical composition is a plurality of doses, each dose comprising 1.0-3.0 mg DNase, said doses administered at least twice, 2-3 times, 2-4 times or 2-6 times daily.