Preparation of samples for analysis and sampling device therefor
A sampling device comprising a body that defines a fluid flow path from an inlet opening, wherein the flow path includes a bed of a porous polymer monolith selected to adsorb bioparticles from a matrix drawn or dispensed through the inlet opening and the bed.
1. A method of preparing for analysis a matrix containing bioparticles, comprising:
drawing or dispensing a volume of the matrix along a flow path defined by a sampling body through a bed of porous polymer monolith in the flow path, which bed is of sufficient length along the flow path whereby the bed adsorbs the bioparticles and a sub-volume of the matrix substantially free of the bioparticles remains adjacent to the bioparticles in the sampling body; and
recovering matrix from the sub-volume for analysis.
2. A method according to claim 1 wherein said body is a tubular body and the bed is a plug of the porous polymer monolith that fills a cross-section of the flow path.
3. A method according to claim 2 wherein the porous polymer monolith is a result of polymerisation of two or more monomers in the presence of two or more porogens.
4. A method according to claim 1 including forming the bed in situ by initiation of a polymerisation mixture within said body.
5. A method according to claim 4 wherein said initiation is by ultraviolet radiation.
6. A method according to claim 1 wherein the porous polymer monolith is a result of polymerisation of two or more monomers in the presence of two or more porogens.
7. A method according to claim 6 wherein the monomers are methacrylates and the porogens include an alcohol and an alkane.
8. A method according to claim 1 wherein the porous monomer monolith is selected to adsorb at least red blood cells from whole or buffered blood.
9. A method according to claim 8 wherein the porous polymer monolith is selected to additionally adsorb white blood cells and platelets from the whole or buffered blood whereby to obtain a cell-free plasma or plasma-like fraction.