Truncated secretory aspartyl proteinase 2
The present invention relates to an isolated truncated form of the secretory aspartyl proteinase 2, as well as to nucleic acid molecules encoding same. The present invention also relates to a composition comprising an isolated truncated form of the secretory aspartyl proteinase 2, as well as to nucleic acid molecules encoding same.
1. An immunogenic composition comprising an effective amount of an isolated polypeptide comprising an amino acid sequence at least 95% identical to SEQ ID NO: 1 over the entire length of SEQ ID NO: 1, wherein the polypeptide is fewer than 342 amino acids in length and an effective amount of a heterologous immune-stimulatory protein, wherein the immunogenic composition is formulated as a sterile solution.
2. The immunogenic composition of claim 1 , wherein the amino acid sequence of the isolated polypeptide is at least 96% identical to SEQ ID NO: 1 over the entire length of SEQ ID NO: 1.
3. The immunogenic composition of claim 1 , wherein the amino acid sequence of the isolated polypeptide is at least 97% identical to SEQ ID NO: 1 over the entire length of SEQ ID NO: 1.
4. The immunogenic composition of claim 1 , wherein the amino acid sequence of the isolated polypeptide is at least 98% identical to SEQ ID NO: 1 over the entire length of SEQ ID NO: 1.
5. The immunogenic composition of claim 1 , wherein the amino acid sequence of the isolated polypeptide is at least 99% identical to SEQ ID NO: 1 over the entire length of SEQ ID NO: 1.
6. The immunogenic composition of any one of claims 1 to 5 further comprising an excipient, a diluent, or a delivery vehicle.
7. The immunogenic composition of claim 6 wherein the delivery vehicle is a virosome.
8. The immunogenic composition of claim 7 , wherein the isolated polypeptide is linked to the surface of the virosome.
9. The immunogenic composition of claim 7 , wherein the isolated polypeptide is contained in the lumen of the virosome.
10. The immunogenic composition of claim 7 , the isolated polypeptide is both linked to the surface of the virosome and contained in the lumen of the virosome.
11. The immunogenic composition of claim 1 , wherein the amino acid sequence of the isolated polypeptide consists of an amino acid sequence at least 95% identical to SEQ ID NO: 1 over the entire length of SEQ ID NO: 1.
12. The immunogenic composition of claim 1 , wherein the immune-stimulatory protein is selected from the group consisting of cholera toxin, Escherichia coli heat-labile toxin, cytokine, and chemokine.
13. The immunogenic composition of claim 12 , wherein the cytokine is selected from the group consisting of interleukin-2 (IL-2), interleukin (IL-12) and granulocyte macrophage colony-stimulating factor (GM-CSF).
14. A kit comprising the immunogenic composition of claim 1 .
15. A method of eliciting an immune response in a mammal against Candida albicans comprising administering to the mammal an effective amount of the immunogenic composition of claim 1 .
16. The method of claim 15 , wherein the immunogenic composition further comprises an excipient, a diluent, or a delivery vehicle.
17. The method of claim 16 , wherein the delivery vehicle is a virosome.
18. The method of claim 17 , wherein the isolated polypeptide is linked to the surface of the virosome.
19. The method of claim 17 , wherein the isolated polypeptide is contained in the lumen of the virosome.
20. The method of claim 17 , the isolated polypeptide is both linked to the surface of the virosome and contained in the lumen of the virosome.