Mutant sodium channel Nav1.7 and methods related thereto
Described are mutant Na v 1.7 sodium channel alpha-subunits and nucleic acid sequences encoding such mutants. Further described are methods for characterizing a nucleic acid sequence that encodes a Na v 1.7 sodium channel alpha-subunit, methods for determining a Na v 1.7 haplotype, methods for determining a subject's predisposition to a neurologic disorder associated with a sodium channel mutation, and methods of identifying a compound that modulates mutant Na v 1.7 sodium channels. Other materials, compositions, articles, devices, and methods relating to mutant Na v 1.7 sodium channels are also described herein.
1. An expression vector comprising an isolated nucleic acid that encodes an amino acid comprising the sequence set forth in any of SEQ ID NOs: 79, 80, 81, 82, 83, 84, or 85 operably linked to an expression control sequence, wherein the isolated nucleic acid is heterologous to the expression vector.
2. The expression vector of claim 1 , wherein the isolated nucleic acid comprises the sequence as set forth in any of SEQ ID NOs: 86, 87, 88, 89, 90, 91, or 92.
3. A cultured cell comprising the vector of claim 1 .
4. A method of making a mutant Nav1.7 sodium channel alpha subunit comprising culturing the cell of claim 3 under conditions allowing expression of the polypeptide encoded by the nucleic acid, wherein the polypeptide comprises a mutant Nav1.7 sodium channel.