Polypeptides for use in self-assembling protein nanostructures
Synthetic nanostructures, polypeptides that are useful, for example, in making synthetic nanostructures, and methods for using such synthetic nanostructures are disclosed herein.
1. An isolated polypeptide comprising an amino acid sequence that is at least 75% identical over its length, and identical at least at one identified interface position, to the amino acid sequence of a polypeptide selected from the group consisting of SEQ ID NOS:1-34:
Identified
interface
Name
Amino Acid Sequence
residues
I53-34A
MEGMDPLAVLAESRLLPLLTVRGGE
I53-34A:
SEQ ID
DLAGLATVLELMGVGALEITLRTEK
28,32,36,
NO: 1
GLEALKALRKSGLLLGAGTVRSPKE
37,186,
AEAALEAGAAFLVSPGLLEEVAALA
188,191,
QARGVPYLPGVLTPTEVERALALGL
192,195
SALKFFPAEPFQGVRVLRAYAEVFP
EVRFLPTGGIKEEHLPHYAALPNLL
AVGGSWLLQGDLAAVMKKVKAAKAL
LSPQAPG
I53-34B
MTKKVGIVDTTFARVDMAEAAIRTL
I53-34B:
SEQ ID
KALSPNIKIIRKTVPGIKDLPVACK
19,20,23,
NO: 2
KLLEEEGCDIVMALGMPGKAEKDKV
24,27,
CAHEASLGLMLAQLMTNKHIIEVFV
109,113,
HEDEAKDDDELDILALVRAIEHAAN
116,117,
VYYLLFKPEYLTRMAGKGLRQGRED
120,124,
AGPARE
148
I53-40A
MTKKVGIVDTTFARVDMASAAILTL
I53-40A:
SEQ ID
KMESPNIKIIRKTVPGIKDLPVACK
20,23,24,
NO: 3
KLLEEEGCDIVMALGMPGKAEKDKV
27,28,
CAHEASLGLMLAQLMTNKHIIEVFV
109,112,
HEDEAKDDAELKILAARRAIEHALN
113,116,
VYYLLFKPEYLTRMAGKGLRQGFED
120,124
AGPARE
I53-40B
MSTINNQLKALKVIPVIAIDNAEDI
I53-40B:
SEQ ID
IPLGKVLAENGLPAAEITFRSSAAV
47,51,54,
NO: 4
KAIMLLRSAQPEMLIGAGTILNGVQ
58,74,102
ALAAKEAGATFVVSPGFNPNTVRAC
QIIGIDIVPGVNNPSTVEAALEMGL
TTLKFFPAEASGGISMVKSLVGPYG
DIRLMPTGGITPSNIDNYLAIPQVL
ACGGTWMVDKKLVTNGEWDEIARLT
REIVEQVNP
I53-47A
MPIFTLNTNIKATDVPSDFLSLTSR
I53-47A:
SEQ ID
LVGLILSKPGSYVAVHINTDQQLSF
22,25,29,
NO: 5
GGSTNPAAFGTLMSIGGIEPSKNRD
72,79,86,
HSAVLFDHLNAMLGIPKNRMYIHFV
87
NLNGDDVGWNGTTF
I53-47B
MNQHSHKDYETVRIAVVRARWHADI
I53-47B:
SEQ ID
VDACVEAFEIAMAAIGGDRFAVDVF
28,31,35,
NO: 6
DVPGAYEIPLHARTLAETGRYGAVL
36,39,
GTAFVVNGGIYRHEFVASAVIDGMM
131,132,
NVQLSTGVPVLSAVLTPHRYRDSAE
135,139,
HHRFFAAHFAVKGVEAARACIEILA
146
AREKIAA
I53-50A
MKMEELFKKHKIVAVLRANSVEEAI
I53-50A:
SEQ ID
EKAVAVFAGGVHLIEITFTVPDADT
25,29,33,
NO: 7
VIKALSVLKEKGAIIGAGTVTSVEQ
54,57
CRKAVESGAEFIVSPHLDEEISQFC
KEKGVFYMPGVMTPTELVKAMKLGH
TILKLFPGEVVGPQEVKAMKGPFPN
VKEVPTGGVNLDNVCEWEKAGVLAV
GVGSALVKGTPDEVREKAKAFVEKI
RGCTE
I53-50B
MNQHSHKDYETVRIAVVRARWHAEI
I53-50B:
SEQ ID
VDACVSAFEAAMADIGGDRFAVDVE
24,28,36,
NO: 8
DVPGAYEIPLHARTLAETGRYGAVL
124,125,
GTAFVVNGGIYRHEEVASAVIDGMM
127,128,
NVQLSTGVPVLSAVLTPHRYRDSDA
129,131,
HTLLFLALFAVKGMEAARACVEILA
132,133,
AREKIAA
135,139
I53-51A
MFTKSGDDGNTNVINKRVGKDSPLV
I53-51A:
SEQ ID
NFLGDLDELNSFIGFAISKIPWEDM
80,83,86,
NO: 9
KKDLERVQVELFEIGEDLSTQSSKK
87,88,90,
KIDESYVLWLLAATAIYRIESGPVK
91,94,
LFVIPGGSEEASVLHVTRSVARRVE
166,172,
RNAVKYTKELPEINRMIIVYLNRLS
176
SLLFAMALVANKRRNQSEKIYEIGK
SW
I53-51B
MNQHSHKDYETVRIAVVRARWHADI
I53-51B:
SEQ ID
VDQCVRAFEEAMADAGGDRFAVDVF
31,35,36,
NO: 10
DVPGAYEIPLHARTLAETGRYGAVL
40,122,
GTAFVVNGGIYRHEFVASAVIDGMM
124,128,
NVQLSTGVPVLSAVLTPHRYRSSRE
131,135,
HHEFFREHFMVKGVEAAAACITILA
139,143,
AREKIAA
146,147
I52-03A
MGHTKGPTPQQHDGSALRIGWHARW
I52-03A:
SEQ ID
NKTIIMPLLIGTIAKLLECGVKASN
28,32,36,
NO: 11
IVVQSVPGSWELPIAVQRLYSASQL
39,44,49
QTPSSGPSLSAGDLLGSSTTDLTAL
PTTTASSTGPFDALIAIGVLIKGET
MHFEYIADSVSHGLMRVQLDTGVPV
IFGVLTVLTDDQAKARAGVIEGSHN
HGEDWGLAAVEMGVRRRDWAAGKTE
I52-03B
MYEVDHADVYDLFYLGRGKDYAAEA
I52-03B:
SEQ ID
SDIADLVRSRTPEASSLLDVACGTG
94,115,
NO: 12
THLEHFTKEFGDTAGLELSEDMLTH
116,206,
ARKRLPDATLHQGDMRDFQLGRKFS
213
AVVSMFSSVGYLKTVAELGAAVASF
AEHLEPGGVVVVEPWWFPETFADGW
VSADVVRRDGRTVARVSHSVREGNA
TRMEVHFTVADPGKGVRHFSDVHLI
TLFHQREYEAAFMAAGLRVEYLEGG
PSGRGLFVGVPA
I52-32A
MGMKEKFVLIITHGDFGKGLLSGAE
I52-32A:
SEQ ID
VIIGKQENVHTVGLNLGDNIEKVAK
47,49,53,
NO: 13
EVMRIIIAKLAEDKEIIIVVDLFGG
54,57,58,
SPFNIALEMMKTFDVKVITGINMPM
61,83,87,
LVELLTSINVYDTTELLENISKIGK
88
DGIKVIEKSSLKM
I52-32B
MKYDGSKLRIGILHARWNLEIIAAL
I52-32B:
SEQ ID
VAGAIKRLQEFGVKAENIIIETVPG
19,20,23,
NO: 14
SFELPYGSKLFVEKQKRLGKPLDAI
30,40
IPIGVLIKGSTMHFEYICDSTTHQL
MKLNFELGIPVIFGVLTCLTDEQAE
ARAGLIEGKMHNHGEDWGAAAVEMA
TKFN
I52-33A
MAVKGLGEVDQKYDGSKLRIGILHA
I52-33A:
SEQ ID
RWNRKIILALVAGAVLRLLEFGVKA
33,41,44,
NO: 15
ENIIIETVPGSFELPYGSKLFVEKQ
50
KRLGKPLDAIIPIGVLIKGSTMHFE
YICDSTTHQLMKLNFELGIPVIFGV
LTCLTDEQAEARAGLIEGKMHNHGE
DWGAAAVEMATKFN
I52-33B
MGANWYLDNESSRLSFTSTKNADIA
I52-33B:
SEQ ID
EVHRFLVLHGKVDPKGLAEVEVETE
61,63,66,
NO: 16
SISTGIPLRDMLLRVLVFQVSKFPV
67,72,
AQINAQLDMRPINNLAPGAQLELRL
147,148,
PLTVSLRGKSHSYNAELLATRLDER
154,155
RFQVVTLEPLVIHAQDFDMVRAFNA
LRLVAGLSAVSLSVPVGAVLIFTAR
I32-06A
MTDYIRDGSAIKALSFAIILAEADL
I32-06A:
SEQ ID
RHIPQDLQRLAVRVIHACGMVDVAN
9,12,13,
NO: 17
DLAFSEGAGKAGRNALLAGAPILCD
14,20,30,
ARMVAEGITRSRLPADNRVIYTLSD
33,34
PSVPELAKKIGNTRSAAALDLWLPH
IEGSIVAIGNAPTALFRLFELLDAG
APKPALIIGMPVGFVGAAESKDELA
ANSRGVPYVIVRGRRGGSAMTAAAV
NALASERE
I32-06B
MITVFGLKSKLAPRREKLAEVIYSS
I32-06B:
SEQ ID
LHLGLDIPKGKHAIRFLCLEKEDFY
24,71,73,
NO: 18
YPFDRSDDYTVIEINLMAGRSEETK
76,77,80,
MLLIFLLFIALERKLGIRAHDVEIT
81,84,85,
IKEQPAHCWGFRGRTGDSARDLDYD
88,114,
IYV
118
I32-19A
MGSDLQKLQRFSTCDISDGLLNVYN
I32-19A:
SEQ ID
IPTGGYFPNLTAISPPQNSSIVGTA
208,213,
NO: 19
YTVLFAPIDDPRPAVNYIDSVPPNS
218,222,
ILVLALEPHLQSQFHPFIKITQAMY
225,226,
GGLMSTRAQYLKSNGTVVFGRIRDV
229,233
DEHRTLNHPVFAYGVGSCAPKAVVK
AVGTNVQLKILTSDGVTQTICPGDY
IAGDNNGIVRIPVQETDISKLVTYI
EKSIEVDRLVSEAIKNGLPAKAAQT
ARRMVLKDYI
I32-19B
MSGMRVYLGADHAGYELKQAIIAFL
I32-19B:
SEQ ID
KMTGHEPIDCGALRYDADDDYPAFC
20,23,24,
NO: 20
IAAATRTVADPGSLGIVLGGSGNGE
27,117,
QIAANKVPGARCALAWSVQTAALAR
118,122,
EHNNAQLIGIGGRMHTLEEALRIVK
125
AFVTTPWSKAQRHQRRIDILAEYER
THEAPPVPGAPA
I32-28A
MGDDARIAAIGDVDELNSQIGVLLA
I32-28A:
SEQ ID
EPLPDDVRAALSAIQHDLFDLGGEL
60,61,64,
NO: 21
CIPGHAAITEDHLLRLALWLVHYNG
67,68,71,
QLPPLEEFILPGGARGAALAHVCRT
110,120,
VCRRAERSIKALGASEPLNIAPAAY
123,124,
VNLLSDLLFVLARVLNRAAGGADVL
128
WDRTRAH
I32-28B
MILSAEQSFTLRHPHGQAAALAFVR
I32-28B:
SEQ ID
EPAAALAGVQRLRGLDSDGEQVWGE
35,36,54,
NO: 22
LLVRVPLLGEVDLPFRSEIVRTPQG
122,129,
AELRPLTLTGERAWVAVSGQATAAE
137,140,
GGEMAFAFQFQAHLATPEAEGEGGA
141,144,
AFEVMVQAAAGVTLLLVAMALPQGL
148
AAGLPPA
I53-40A.1
MTKKVGIVDTTFARVDMASAAILTL
I53-40A:
SEQ ID
KMESPNIKIIRKTVPGIKDLPVACK
20,23,24,
NO: 23
KLLEEEGCDIVMALGMPGKKEKDKV
27,28,
CAHEASLGLMLAQLMTNKHIIEVFV
109,112,
HEDEAKDDAELKILAARRAIEHALN
113,116,
VYYLLFKPEYLTRMAGKGLRQGFED
120,124
AGPARE
I53-40B.1
MDDINNQLKRLKVIPVIAIDNAEDI
I53-40B:
SEQ ID
IPLGKVLAENGLPAAEITERSSAAV
47,51,54,
NO: 24
KAIMLLRSAQPEMLIGAGTILNGVQ
58,74,102
ALAAKEAGADFVVSPGFNPNTVRAC
QIIGIDIVPGVNNPSTVEQALEMGL
TTLKFFPAEASGGISMVKSLVGPYG
DIRLMPTGGITPDNIDNYLAIPQVL
ACGGTWMVDKKLVRNGEWDEIARLT
REIVEQVNP
I53-47A.1
MPIFTLNTNIKADDVPSDFLSLTSR
I53-47A:
SEQ ID
LVGLILSKPGSYVAVHINTDQQLSF
22,25,29,
NO: 25
GGSTNPAAFGTLMSIGGIEPDKNRD
72,79,86,
HSAVLFDHLNAMLGIPKNRMYIHFV
87
NLNGDDVGWNGTTF
I53-47A.1
MPIFTLNTNIKADDVPSDFLSLTSR
I53-47A:
NegT2
LVGLILSEPGSYVAVHINTDQQLSF
22,25,29,
SEQ ID
GGSTNPAAFGTLMSIGGIEPDKNED
72,79,86,
NO: 26
HSAVLFDHLNAMLGIPKNRMYIHFV
87
DLDGDDVGWNGTTF
I53-47B.1
MNQHSHKDHETVRIAVVRARWHADI
I53-47B:
SEQ ID
VDACVEAFEIAMAAIGGDRFAVDVF
28,31,35,
NO: 27
DVPGAYEIPLHARTLAETGRYGAVL
36,39,
GTAFVVNGGIYRHEFVASAVIDGMM
131,132,
NVQLDTGVPVLSAVLTPHRYRDSDE
135,139,
HHRFFAAHFAVKGVEAARACIEILN
146
AREKIAA
I53-47B.1
MNQHSHKDHETVRIAVVRARWHADI
I53-47B:
NegT2
VDACVEAFEIAMAAIGGDRFAVDVE
28,31,35,
SEQ ID
DVPGAYEIPLHARTLAETGRYGAVL
36,39,
NO: 28
GTAFVVDGGIYDHEEVASAVIDGMM
131,132,
NVQLDTGVPVLSAVLTPHEYEDSDE
135,139,
DHEFFAAHFAVKGVEAARACIEILN
146
AREKIAA
I53-50A.1
MKMEELFKKHKIVAVLRANSVEEAI
I53-50A:
SEQ ID
EKAVAVFAGGVHLIEITFTVPDADT
25,29,33,
NO: 29
VIKALSVLKEKGAIIGAGTVTSVEQ
54,57
CRKAVESGAEFIVSPHLDEEISQFC
KEKGVFYMPGVMTPTELVKAMKLGH
DILKLFPGEVVGPQFVKAMKGPFPN
VKFVPTGGVNLDNVCEWFKAGVLAV
GVGDALVKGDPDEVREKAKKFVEKI
RGCTE
I53-50A.1
MKMEELFKKHKIVAVLRANSVEEAI
I53-50A:
NegT2
EKAVAVFAGGVHLIEITFTVPDADT
25,29,33,
SEQ ID
VIKALSVLKEKGAIIGAGTVTSVEQ
54,57
NO: 30
CRKAVESGAEFIVSPHLDEEISQFC
KEKGVFYMPGVMTPTELVKAMKLGH
DILKLFPGEVVGPEFVEAMKGPFPN
VKFVPTGGVDLDDVCEWFDAGVLAV
GVGDALVEGDPDEVREDAKEFVEEI
RGCTE
I53-50A.1
MKMEELFKKHKIVAVLRANSVEEAI
I53-50A:
PosT1
EKAVAVFAGGVHLIEITFTVPDADT
25,29,33,
SEQ ID
VIKALSVLKEKGAIIGAGTVTSVEQ
54,57
NO: 31
CRKAVESGAEFIVSPHLDEEISQFC
KEKGVFYMPGVMTPTELVKAMKLGH
DILKLFPGEVVGPQFVKAMKGPFPN
VKFVPTGGVNLDNVCKWFKAGVLAV
GVGKALVKGKPDEVREKAKKFVKKI
RGCTE
I53-50B.1
MNQHSHKDHETVRIAVVRARWHAEI
I53-50B:
SEQ ID
VDACVSAFEAAMRDIGGDRFAVDVF
24,28,36,
NO: 32
DVPGAYEIPLHARTLAETGRYGAVL
124,125,
GTAFVVNGGIYRHEFVASAVIDGMM
127,128,
NVQLDTGVPVLSAVLTPHRYRDSDA
129,131,
HTLLFLALFAVKGMEAARACVEILA
132,133,
AREKIAA
135,139
I53-50B.1
MNQHSHKDHETVRIAVVRARWHAEI
I53-50B:
NegT2
VDACVSAFEAAMRDIGGDRFAVDVF
24,28,36,
SEQ ID
DVPGAYEIPLHARTLAETGRYGAVL
124,125,
NO: 33
GTAFVVDGGIYDHEFVASAVIDGMM
127,128,
NVQLDTGVPVLSAVLTPHEYEDSDA
129,131,
DTLLFLALFAVKGMEAARACVEILA
132,133,
AREKIAA
135,139
I53-50B.4
MNQHSHKDHETVRIAVVRARWHAEI
I53-50B:
Pos1
VDACVSAFEAAMRDIGGDRFTAVDV
24,28,36,
SEQ ID
FDVPGAYEIPLHARTLAETGRYGAV
124,125,
NO: 34
LGTAFVVNGGIYRHEFVASAVINGM
127,128,
MNVQLNTGVPVLSAVLTPHNYDKSK
129,131,
AHTLLFLALFAVKGMEAARACVEIL
132,133,
AAREKIAA
135,139
2. The isolated polypeptide of claim 1 , wherein the polypeptide is identical at least at half of the identified interface positions to the amino acid sequence of a polypeptide selected from the group consisting of SEQ ID NO:1-34.
3. The isolated polypeptide of claim 1 , wherein the polypeptide is identical at all of the identified interface positions to the amino acid sequence of a polypeptide selected from the group consisting of SEQ ID NO:1-34.
4. The isolated polypeptide of claim 1 , wherein the polypeptide comprises an amino acid sequence that is at least 90% identical over its length to the sequence of a polypeptide selected from the group consisting of SEQ ID NO:1-34.
5. The isolated polypeptide of claim 1 , wherein the polypeptide comprises the amino acid sequence of a polypeptide selected from the group consisting of SEQ ID NOS: 1-40.
6. A nanostructure, comprising:
(a) a plurality of first assemblies, each first assembly comprising a plurality of identical first polypeptides, wherein the first polypeptides comprise the polypeptide of claim 1 ; and
(b) a plurality of second assemblies, each second assembly comprising a plurality of identical second polypeptides, wherein the second polypeptides comprise the polypeptide of claim 1 , and wherein the second polypeptide differs from the first polypeptide;
wherein the plurality of first assemblies non-covalently interact with the plurality of second assemblies to form a nanostructure.
7. The nanostructure of claim 6 , wherein the nanostructure has icosahedral symmetry.
8. The nanostructure of claim 6 , wherein the first polypeptides and the second polypeptides comprise polypeptides with the amino acid sequences selected from the following pairs:
(i) SEQ ID NO:1 and SEQ ID NO:2 (I53-34A and I53-34B);
(ii) SEQ ID NO:3 and SEQ ID NO:4 (I53-40A and I53-40B);
(iii) SEQ ID NO:3 and SEQ ID NO:24 (I53-40A and I53-40B.1);
(iv) SEQ ID NO:23 and SEQ ID NO:4 (I53-40A.1 and I53-40B);
(v) SEQ ID NO:35 and SEQ ID NO:36 (I53-40A genus and I53-40B genus);
(vi) SEQ ID NO:5 and SEQ ID NO:6 (I53-47A and I53-47B);
(vii) SEQ ID NO:5 and SEQ ID NO:27 (I53-47A and I53-47B.1);
(viii) SEQ ID NO:5 and SEQ ID NO:28 (I53-47A and I53-47B.1NegT2);
(ix) SEQ ID NO:25 and SEQ ID NO:6 (I53-47A.1 and I53-47B);
(x) SEQ ID NO:25 and SEQ ID NO:27 (I53-47A.1 and I53-47B.1);
(xi) SEQ ID NO:25 and SEQ ID NO:28 (I53-47A.1 and I53-47B.1NegT2);
(xii) SEQ ID NO:26 and SEQ ID NO:6 (I53-47A.1NegT2 and I53-47B);
(xiii) SEQ ID NO:26 and SEQ ID NO:27 (I53-47A.1NegT2 and I53-47B.1);
(xiv) SEQ ID NO:26 and SEQ ID NO:28 (I53-47A.1NegT2 and I53-47B.1NegT2);
(xv) SEQ ID NO:37 and SEQ ID NO:38 (I53-47A genus and I53-47B genus);
(xvi) SEQ ID NO:7 and SEQ ID NO:8 (I53-50A and I53-50B);
(xvii) SEQ ID NO:7 and SEQ ID NO:32 (I53-50A and I53-50B.1);
(xix) SEQ ID NO:7 and SEQ ID NO:33 (I53-50A and I53-50B.1NegT2);
(xx) SEQ ID NO:7 and SEQ ID NO:34 (I53-50A and I53-50B.4PosT1);
(xxi) SEQ ID NO:29 and SEQ ID NO:8 (I53-50A.1 and I53-50B);
(xxii) SEQ ID NO:29 and SEQ ID NO:32 (I53-50A.1 and I53-50B.1);
(xxiii) SEQ ID NO:29 and SEQ ID NO:33 (I53-50A.1 and I53-50B.1NegT2);
(xxiv) SEQ ID NO:29 and SEQ ID NO:34 (I53-50A.1 and I53-50B.4PosT1);
(xxv) SEQ ID NO:30 and SEQ ID NO:8 (I53-50A.1NegT2 and I53-50B);
(xxvi) SEQ ID NO:30 and SEQ ID NO:32 (I53-50A.1NegT2 and I53-50B.1);
(xxvii) SEQ ID NO:30 and SEQ ID NO:33 (I53-50A.1NegT2 and I53-50B.1NegT2);
(xxviii) SEQ ID NO:30 and SEQ ID NO:34 (I53-50A.1NegT2 and I53-50B.4PosT1);
(xxix) SEQ ID NO:31 and SEQ ID NO:8 (I53-50A.1PosT1 and I53-50B);
(xxx) SEQ ID NO:31 and SEQ ID NO:32 (I53-50A.1PosT1 and I53-50B.1);
(xxxi) SEQ ID NO:31 and SEQ ID NO:33 (I53-50A.1PosT1 and I53-50B.1NegT2);
(xxxii) SEQ ID NO:31 and SEQ ID NO:34 (I53-50A.1PosT1 and I53-50B.4PosT1);
(xxxiii) SEQ ID NO:39 and SEQ ID NO:40 (I53-50A genus and I53-50B genus);
(xxxiv) SEQ ID NO:9 and SEQ ID NO:10 (I53-51A and I53-51B);
(xxxv) SEQ ID NO:11 and SEQ ID NO:12 (I52-03A and I52-03B);
(xxxvi) SEQ ID NO:13 and SEQ ID NO:14 (I52-32A and I52-32B);
(xxxv) SEQ ID NO:15 and SEQ ID NO:16 (I52-33A and I52-33B)
(xxxvi) SEQ ID NO:17 and SEQ ID NO:18 (I32-06A and I32-06B);
(xxxvii) SEQ ID NO:19 and SEQ ID NO:20 (I32-19A and I32-19B);
(xxxviii) SEQ ID NO:21 and SEQ ID NO:22 (I32-28A and I32-28B); and
(xxxix) SEQ ID NO:23 and SEQ ID NO:24 (I53-40A.1 and I53-40B.1).
9. The nanostructure of claim 6 , wherein the number of identical first polypeptides in each first assembly is different than the number of identical second polypeptides in each second assembly.
10. The nanostructure of claim 9 , wherein each first assembly comprises five copies of the identical first polypeptide, and each second assembly comprises three copies of the identical second polypeptide.
11. The nanostructure of claim 10 , wherein the nanostructure comprises twelve first assemblies and twenty second assemblies.
12. The nanostructure of claim 9 , wherein each first assembly comprises three copies of the identical first polypeptide, and each second assembly comprises two copies of the identical second polypeptide.
13. The nanostructure of claim 12 , wherein the nanostructure comprises twenty first assemblies and thirty second assemblies.
14. The nanostructure of claim 9 , wherein each first assembly comprises five copies of the identical first polypeptide, and each second assembly comprises two copies of the identical second polypeptide.
15. The nanostructure of claim 14 , wherein the nanostructure comprises twelve first assemblies and thirty second assemblies.
16. The nanostructure of claim 6 , further comprising a cargo within the nanostructure.
17. The nanostructure of claim 16 , wherein the cargo comprises a nucleic acid.