Polynucleotides and polypeptides involved in plant fiber development and methods of using same
Isolated polynucleotides are provided. Each of the isolated polynucleotides comprise a nucleic acid sequence encoding a polypeptide having an amino acid sequence at least 80% homologous to SEQ ID NOs:130-258 and 536-791, wherein the polypeptide is capable of regulating cotton fiber development. Also provided are methods of using such polynucleotides for improving fiber quality and/or yield of a fiber producing plant, as well as methods of using such polynucleotides for producing plants having increased biomass/vigor/yield.
1. A nucleic acid construct comprising an isolated polynucleotide comprising a nucleic acid sequence encoding the polypeptide set forth by SEQ ID NO: 931, and a heterologous regulatory sequence which regulates the expression of said polynucleotide sequence in a cell, wherein said heterologous regulatory sequence is set forth by SEQ ID NO:851, wherein said isolated polynucleotide is operably linked to said heterologous regulatory sequence.
2. A transgenic cell comprising the nucleic acid construct of claim 1 .
3. A transgenic plant comprising the nucleic acid construct of claim 1 .
4. A method of generating a transgenic plant, comprising transforming a cell of a plant with the nucleic acid construct of claim 1 , thereby generating the transgenic plant.
5. A method of producing cotton fibers, the method comprising:
(a) generating a transgenic cotton plant transformed with the nucleic acid construct of claim 1 ; and
(b) harvesting the fibers of said transgenic cotton plant, thereby producing the cotton fibers.
6. A method of increasing fiber length of a plant, the method comprising transforming the plant with a nucleic acid construct comprising an isolated polynucleotide comprising a nucleic acid sequence encoding the polypeptide set forth by SEQ ID NO:931 and a heterologous regulatory sequence which regulates the expression of said polynucleotide sequence in a cell, and wherein said isolated polynucleotide is operably linked to said heterologous regulatory sequence, thereby increasing the fiber length of the plant.
7. The method of claim 6 , wherein said plant is selected from the group consisting of cotton, silk cotton tree, desert willow, creosote bush, winterfat, balsa, ramie, kenaf, hemp, roselle, jute, sisal abaca and flax.
8. The method of claim 6 , wherein said regulatory sequence is shorter than 1800 bp.