AAV vectors targeted to oligodendrocytes
The invention relates to chimeric AAV capsids targeted to oligodendrocytes, nucleic acids encoding the capsids, virus vectors and particles comprising the same, methods of producing the vectors, and methods of using the vectors to target oligodendrocytes. The invention further relates to methods of treating a disorder associated with oligodendrocyte dysfunction using the vectors.
1. An adeno-associated virus (AAV) capsid comprising the amino acid sequence of one of SEQ ID NOS:2-4, wherein 25 or fewer amino acids are substituted, deleted, and/or inserted.
2. The AAV capsid of claim 1 covalently linked, bound to, or encapsidating a compound selected from the group consisting of a DNA molecule, an RNA molecule, a polypeptide, a carbohydrate, a lipid, and a small organic molecule.
3. An AAV particle comprising:
an AAV vector genome; and
the AAV capsid of claim 1 , wherein the AAV capsid encapsidates the AAV vector genome.
4. The AAV particle of claim 3 , wherein the AAV vector genome comprises a heterologous nucleic acid.
5. The AAV particle of claim 4 , wherein the heterologous nucleic acid is operably linked to a constitutive promoter.
6. The AAV particle of claim 4 , wherein the heterologous nucleic acid is operably linked to an oligodendrocyte-specific or oligodendrocyte-preferred promoter.
7. A pharmaceutical formulation comprising the AAV particle of claim 3 in a pharmaceutically acceptable carrier.
8. The AAV capsid of claim 1 , wherein 10 or fewer amino acids are substituted, deleted, and/or inserted.
9. The AAV capsid of claim 1 , comprising the amino acid sequence of one of SEQ ID NOS:2-4.