HIV peptides and immunogenic compositions
The present specification discloses an immunogenic composition comprising polypeptide, wherein each of the polypeptides has no more than 100 amino acids, which polypeptides comprises one or more sequences having at least 60% homology with any of SEQ ID 1-4, or comprises two or more epitopes having 7 amino acids or more, each epitope having at least 60% homology with a sub-sequence of any of SEQ ID 1-4 that has the same length as the epitope, wherein, the polypeptide is immunogenic in a vertebrate expressing a major histocompatibility complex (MHC) allele, and wherein the polypeptide is not a complete HIV virus protein.
1. An isolated polypeptide having no more than 50 amino acids, the isolated polypeptide comprising SEQ ID NO: 2,
wherein the polypeptide elicits an immune response in vertebrates against a HIV protein comprising SEQ ID NO: 2.
2. The isolated polypeptide according to claim 1 , wherein the isolated polypeptide comprises a cytotoxic T lymphocyte (CTL), CD 8+ T cell and/or CD4+ T cell epitope.
3. The isolated polypeptide according to claim 2 , wherein the cytotoxic T lymphocyte (CTL), CD 8+ T cell and/or CD4+ T cell epitope has 8, 9 10 or 11 amino acids or more.
4. The isolated polypeptide according to claim 1 , wherein the isolated polypeptide elicits an immune response against a single HIV strain or against a plurality of HIV strains.
5. A polypeptide composition comprising the isolated polypeptide of claim 1 .
6. The polypeptide composition according to claim 5 , wherein the polypeptide comprises a cytotoxic T lymphocyte (CTL), CD 8+ T cell and/or CD4+ T cell epitope.
7. The polypeptide composition according to claim 6 , wherein the cytotoxic T lymphocyte (CTL), CD 8+ T cell and/or CD4+ T cell epitope has 8, 9 10 or 11 amino acids or more.
8. The polypeptide composition of claim 5 , wherein the isolated polypeptide elicits an immune response against a single HIV strain or against a plurality of HIV strains.
9. The polypeptide composition according to claim 5 , wherein the isolated polypeptide is in an amount of 1 ng to 1 g.
10. The polypeptide composition according to claim 5 , further comprising one or more isolated polypeptides having no more than 50 amino acids, the one or more isolated polypeptides comprising an isolated polypeptide comprising SEQ ID NO: 1, an isolated polypeptide comprising SEQ ID NO: 3 an isolated polypeptide comprising SEQ ID NO: 4, or any combination thereof,
wherein the polypeptide elicits an immune response in vertebrates against a HIV protein comprising SEQ ID NO: 1, SEQ ID NO: 3, or SEQ ID NO: 4.
11. The polypeptide composition according to claim 10 , wherein the one or more isolated polypeptides comprises a cytotoxic T lymphocyte (CTL), CD 8+ T cell and/or CD4+ T cell epitope.
12. The polypeptide composition according to claim 11 , wherein the cytotoxic T lymphocyte (CTL), CD 8+ T cell and/or CD4+ T cell epitope has 8, 9 10 or 11 amino acids or more.
13. The polypeptide composition according to claim 10 , wherein the one or more isolated polypeptides elicit an immune response against a single HIV strain or against a plurality of HIV strains.
14. The polypeptide composition according to claim 10 , comprising:
an isolated polypeptide comprising SEQ ID NO: 1; an isolated polypeptide comprising SEQ ID NO: 2; an isolated polypeptide comprising SEQ ID NO: 3; and an isolated polypeptide comprising SEQ ID NO: 4.
15. The polypeptide composition according to claim 10 , wherein one or more isolated polypeptides is in an amount of 1 ng to 1 g.
16. The polypeptide composition according to claim 5 , further comprising an adjuvant and/or an excipient.
17. An isolated polypeptide having no more than 50 amino acids, the isolated polypeptide comprising SEQ ID NO: 2 or a sequence having 85% or more homology to SEQ ID NO: 2.
18. The isolated polypeptide according to claim 17 , wherein the sequence has 95% or more homology to SEQ ID NO: 2.
19. A polypeptide composition comprising the isolated polypeptide of claim 17 .
20. The polypeptide composition according to claim 19 , wherein the sequence has 95% or more homology to SEQ ID NO: 2.
21. The polypeptide composition according to claim 19 , wherein the isolated polypeptide is in an amount of 1 ng to 1 g.
22. The polypeptide composition according to claim 19 , further comprising one or more isolated polypeptides having no more than 50 amino acids, the one or more isolated polypeptides comprising an isolated polypeptide comprising SEQ ID NO: 1 or a sequence having 85% or more homology to SEQ ID NO: 1, an isolated polypeptide comprising SEQ ID NO: 3 or a sequence having 85% or more homology to SEQ ID NO: 3, an isolated polypeptide comprising SEQ ID NO: 4 or a sequence having 85% or more homology to SEQ ID NO: 4 or any combination thereof.
23. The polypeptide composition according to claim 22 , wherein the one or more isolated polypeptides comprise an isolated polypeptide comprising SEQ ID NO: 1 or a sequence having 85% or more homology to SEQ ID NO: 1, an isolated polypeptide comprising SEQ ID NO: 3 or a sequence having 85% or more homology to SEQ ID NO: 3, and an isolated polypeptide comprising SEQ ID NO: 4 or a sequence having 85% or more homology to SEQ ID NO: 4 or any combination thereof.
24. The polypeptide composition according to claim 22 , wherein the one or more isolated polypeptides comprises an isolated polypeptide comprising SEQ ID NO: 1 or a sequence having 95% or more identity with SEQ ID NO: 1; an isolated polypeptide comprising SEQ ID NO: 2 or a sequence having 95% or more identity with SEQ ID NO: 2; an isolated polypeptide comprising SEQ ID NO: 3 or a sequence having 95% or more identity with SEQ ID NO: 3; or an isolated polypeptide comprising SEQ ID NO: 4 or a sequence having 95% or more identity with SEQ ID NO: 4.
25. The polypeptide composition according to claim 24 , wherein the one or more isolated polypeptides comprise an isolated polypeptide comprising SEQ ID NO: 1 or a sequence having 95% or more homology to SEQ ID NO: 1, an isolated polypeptide comprising SEQ ID NO: 3 or a sequence having 95% or more homology to SEQ ID NO: 3, and an isolated polypeptide comprising SEQ ID NO: 4 or a sequence having 95% or more homology to SEQ ID NO: 4 or any combination thereof.
26. The polypeptide composition according to claim 22 , wherein the one or more isolated polypeptides is in an amount of 1 ng to 1 g.
27. The polypeptide composition according to claim 19 , further comprising an adjuvant and/or an excipient.